Sialylation, Fucosylation & Clinical Glycan Biomarkers — Hands-on

Explore how terminal sialylation and fucosylation signatures reflect inflammation, cancer, immune regulation and biotherapeutic quality. This module links glycobiology concepts to LC-MS based released glycan and glycopeptide workflows, and shows how to derive quantitative sialylation and fucosylation metrics suitable for clinical glycan biomarker panels and CQAs.

Sialylation, Fucosylation & Clinical Glycan Biomarkers

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Session Index

Session 1 — Clinical Glycobiology of Sialylation & Fucosylation Session 2 — Analytical Workflows for Terminal Glycan Features Session 3 — Biomarker Panels, Metrics & Statistics Session 4 — Mini Capstone: Clinical Glycan Signature Panel

Session 1

Fee: Rs 8800

Clinical Glycobiology of Sialylation & Fucosylation

Sialic acid linkages, positions and functional themes

alpha2-3 vs alpha2-6 vs alpha2-8 linkages Neu5Ac vs other sialic acids charge, half life and masking effects

Core and outer arm fucosylation patterns

core fucosylation basics Lewis type motifs and selectins branch and antenna specific fucose

Clinical and biotherapeutic relevance of terminal glycans

cancer and inflammation markers antibody effector function and half life acute phase and metabolic disease signatures

Session 2

Fee: Rs 11800

Analytical Workflows for Terminal Glycan Features

Released glycan workflows focused on sialylation and fucosylation

derivatization strategies for sialic acids stabilizing labile linkages column and gradient choices for terminals

Glycopeptide level views of terminal patterns

site resolved sialylation and fucosylation fragmentation for linkage and position hints multi attribute monitoring perspectives

MS signals and diagnostic ions for terminals

oxonium ions associated with sialylation fucose related fragment patterns checking for desialylation or loss

Session 3

Fee: Rs 14800

Biomarker Panels, Metrics & Statistics

Defining sialylation and fucosylation metrics

sialylation index per protein or sample core vs outer arm fucose ratios galactosylation and antenna context

From quantitative tables to clinical glycan panels

feature selection and composite scores reference ranges and cut offs batch and instrument effect handling

Basic diagnostic and prognostic performance checks

ROC curves and AUC sensitivity, specificity and calibration visual summaries for clinical teams

Session 4

Fee: Rs 18800

Mini Capstone: Clinical Glycan Signature Panel

Build a small sialylation and fucosylation signature on example data

from LC-MS tables to biomarker candidates

Generate visual and tabular outputs for clinical review

panel summary plots and heatmaps simple score or index derivation narrative summary for non specialists

Deliverables and documentation set

curated biomarker table figures ready for slides or reports notes on validation and next steps

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Sialylation, Fucosylation & Clinical Glycan Biomarkers Training | Terminal Glycans & Clinical Panels

Sialylation, Fucosylation & Clinical Glycan Biomarkers — Hands-on