Stable Isotope Tracing & Metabolic Flux Analysis (13C MFA) — Hands-on

Move from static metabolite levels to dynamic pathway fluxes. This module introduces stable isotope tracer concepts, 13C labeling strategies, isotopologue patterns, natural abundance correction, and entry level metabolic flux analysis so that you can design realistic tracer studies and extract quantitative pathway information from LC–MS or GC–MS data.

Stable Isotope Tracing & Metabolic Flux Analysis (13C MFA)

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Session Index

Session 1 — Tracer Concepts & 13C MFA Experimental Design Session 2 — Isotopologues, MID Patterns & Abundance Correction Session 3 — Metabolic Flux Analysis Models & Fitting Concepts Session 4 — Mini Capstone: Simple 13C MFA Pathway Story

Session 1

Fee: Rs 8800

Tracer Concepts & 13C MFA Experimental Design

Why use stable isotopes and what questions 13C MFA can answer

turnover vs pool size pathway contribution ideas flux vs abundance thinking

Common tracers and labeling strategies for 13C studies

U-13C glucose and glutamine concepts single vs multiple tracers steady state vs pulse chase designs

Practical experimental considerations for tracer studies

labeling time and sampling plan medium formulation and background carbon QC, replicates and viability checks

Session 2

Fee: Rs 11800

Isotopologues, MID Patterns & Abundance Correction

From LC–MS / GC–MS peaks to isotopologue distributions

M+0, M+1, M+2 pattern ideas mass resolution and overlap concepts building mass isotopomer distributions (MID)

Natural abundance and isotopic impurity correction (concepts)

why raw labeling can be misleading correction matrix intuition intro to software assisted correction

Expressing labeling patterns in useful summary metrics

fractional enrichment average labeling or mole percent labeled linking MID changes to pathway hypotheses

Session 3

Fee: Rs 14800

Metabolic Flux Analysis Models & Fitting Concepts

From pathways to flux models for 13C MFA

stoichiometric network sketches reversible vs irreversible reactions atom transitions and mapping ideas

EMU style thinking and simulation of labeling patterns (high level)

elementary metabolite units concept reducing model complexity simulated vs observed MID comparison

Flux fitting, identifiability and uncertainty at a conceptual level

objective functions and residuals confidence intervals for fluxes (idea) what can and cannot be concluded

Session 4

Fee: Rs 18800

Mini Capstone: Simple 13C MFA Pathway Story

Interpreting labeling for a small central carbon pathway segment

Theory + Practical

Constructing a simple flux scenario consistent with MID data

qualitative flux diagrams explaining major label routes noting alternative interpretations

Deliverables: tracer design sheet, MID summary & pathway narrative

experiment and tracer design summary (PDF/HTML) corrected MID and enrichment table (CSV/TSV) short 13C MFA interpretation note

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Stable Isotope Tracing & Metabolic Flux Analysis (13C MFA) Training | Tracer Design & Flux Concepts

Stable Isotope Tracing & Metabolic Flux Analysis (13C MFA) — Hands-on