Structure Guided Mutagenesis & Protein Design — Hands-on

Learn how to use 3D structural information to plan, prioritise and interpret protein mutations. This module covers hotspot identification, in silico mutagenesis, stability and binding impact assessment, and multi parameter protein design so that you can propose rational, testable mutations for improved activity, specificity, stability or developability.

Structure Guided Mutagenesis & Protein Design

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Session Index

Session 1 — Principles of Structure Guided Mutagenesis Session 2 — In Silico Mutagenesis & Stability Assessment Session 3 — Modelling Mutants & Functional Impact Session 4 — Design Campaigns, Ranking & Case Study

Session 1

Fee: Rs 8800

Principles of Structure Guided Mutagenesis

From sequence based to structure guided design

rational vs random mutagenesis when structure adds value design objectives and constraints

Identifying structural hotspots for mutation

active and allosteric sites interfaces and binding pockets stability cores and flexible loops

Reading structures for design decisions

contacts, hydrogen bonds and packing solvent exposure and networks leveraging existing mutational data

Session 2

Fee: Rs 11800

In Silico Mutagenesis & Stability Assessment

In silico alanine scanning and saturation mutagenesis

probing contribution of side chains systematic exploration of substitutions interpreting scan results for design

Stability and ddG style calculations for mutants

folding stability predictions binding free energy impact overview limits, error bars and ranking logic

Filtering designs for expression and solubility

charged and polar surface design removing aggregation prone motifs balancing stability with function

Session 3

Fee: Rs 14800

Modelling Mutants & Functional Impact

Building and relaxing mutant models

side chain replacement workflows local minimisation and clean up checking for clashes and distortions

Assessing impact on binding and activity

docking mutants with ligands or partners simple MD based checks of flexibility qualitative mechanism of action hypotheses

Multi parameter design thinking

activity vs stability vs specificity developability considerations trade offs and design envelopes

Session 4

Fee: Rs 18800

Design Campaigns, Ranking & Case Study

End to end structure guided design case study

Theory + Practical

Ranking and selecting variants for experiments

scoring tables and design sheets shortlisting primary and backup designs communicating risk and confidence levels

Reporting and documenting design rationale

figures of before vs after structures clear mechanism and objective statements notebooks and files for reproducibility

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Structure Guided Mutagenesis & Protein Design Training | Rational Protein Engineering

Structure Guided Mutagenesis & Protein Design — Hands-on