Study Design, Sampling & Decontamination Strategies — Hands-on

Learn how to design contamination-aware microbiome and metagenomics studies from first principles. This module covers sampling frames, collection and storage logistics, negative and positive controls, batch structure, and practical decontamination strategies so that downstream analysis is trustworthy and publication ready.

Study Design, Sampling & Decontamination Strategies

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Session Index

Session 1 — Microbiome Study Design Principles Session 2 — Sampling, Storage & Transport Session 3 — Controls, Contaminants & Batch Effects Session 4 — Mini Capstone: Protocol & Metadata Blueprint

Session 1

Fee: Rs 8800

Microbiome Study Design Principles

Translating biological questions into study designs

case control and cohort cross sectional and longitudinal paired designs

Endpoints, inclusion criteria and covariates

primary and secondary endpoints confounders and effect modifiers sample size considerations

Linking design choices to amplicon and shotgun workflows

biological versus technical replicates time points and follow up cost versus depth trade offs

Session 2

Fee: Rs 11800

Sampling, Storage & Transport

Sampling frames and collection strategies

stool, oral, skin, soil, water self collection kits field logistics

Storage conditions and stabilization

cold chain and freeze thaw stabilizing reagents time to processing

Labeling, barcoding and chain of custody

unique sample identifiers tracking sheets linking to metadata

Session 3

Fee: Rs 14800

Controls, Contaminants & Batch Effects

Designing negative and positive controls

field blanks extraction and PCR blanks mock communities

Common sources of contamination

reagents and kits laboratory environment index hopping and carryover

Batch effects, randomization and blocking

plate and run layout technical replicates documentation for correction downstream

Session 4

Fee: Rs 18800

Mini Capstone: Protocol & Metadata Blueprint

Drafting a contamination-aware study protocol

guided template plus review

Sampling schedule, control strategy and logistics plan

visit and collection timetable control allocation lab handover notes

Metadata and documentation checklist

MIxS style fields design tables handover for analysis teams

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Study Design, Sampling & Decontamination Strategies Training | Microbiome & Metagenomics Projects

Study Design, Sampling & Decontamination Strategies — Hands-on