Targeted Metabolomics — Absolute Quantitation & Validation — Hands-on

Move from discovery profiling to rigorous, reportable numbers. This module focuses on targeted metabolomics assay design, internal standard strategy, calibration curve construction, and method validation parameters so that you can deliver absolute concentrations and decision-grade readouts for biomarkers and panels.

Targeted Metabolomics — Absolute Quantitation & Validation

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Session Index

Session 1 — Principles of Targeted Metabolomics & Panel Design Session 2 — Calibration Curves, Internal Standards & LC–MS Methods Session 3 — Method Validation: Linearity, Precision, Accuracy Session 4 — Mini Capstone: Validation Plan & Quant Report Template

Session 1

Fee: Rs 8800

Principles of Targeted Metabolomics & Panel Design

Why and when to move from untargeted to targeted panels

biomarker verification clinical decision support fit-for-purpose validation

Choosing metabolites and matrices for targeted assays

biological rationale dynamic range & abundance matrix complexity & interferences

Overview of LC–MS/MS and MRM / SRM concepts for targeting

precursor / product ions dwell time & cycle time scheduled MRM (concepts)

Session 2

Fee: Rs 11800

Calibration Curves, Internal Standards & LC–MS Methods

Designing calibration curves for absolute quantitation

concentration ranges number of levels & replicates weighting (1/x, 1/x²) concepts

Internal standards and response factor calculations

stable isotope labelled IS class-specific surrogates IS-normalized signals

Translating a targeted panel into an LC–MS/MS method

MRM transition list collision energy & cone voltage chromatographic alignment

Session 3

Fee: Rs 14800

Method Validation: Linearity, Precision, Accuracy

Key validation parameters for targeted metabolomics methods

linearity & R² intra / inter-day precision accuracy & bias

LOD, LOQ, recovery and matrix effects (concepts)

signal-to-noise approaches spike-recovery studies post-column vs post-extraction spike

Stability assessments for standards and real samples

freeze–thaw stability bench-top / autosampler long term storage checks

Session 4

Fee: Rs 18800

Mini Capstone: Validation Plan & Quant Report Template

Drafting a validation plan for a targeted metabolite panel

Theory + Practical

Structuring acceptance criteria and QC rules for routine runs

control charts & QC samples rejection / repeat criteria documentation checklist

Deliverables: validation summary & absolute quant report template

validation plan and summary (PDF/Word) calibration & QC tracking sheet (XLS/CSV) quant results report template

PDF

Targeted Metabolomics — Absolute Quantitation & Validation Training | LC–MS/MS Panels & Method Validation

Targeted Metabolomics — Absolute Quantitation & Validation — Hands-on