Targeted Metabolomics — Standards & Quantitation — Hands-on

Learn how to design, execute and validate targeted metabolomics assays that deliver robust absolute and relative quantitation. This module focuses on standards and internal standards, calibration and QC design, and practical quantitation workflows so you can support clinical, pharma and translational research with publication- and audit-ready data.

Targeted Metabolomics — Standards & Quantitation

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Session Index

Session 1 — Targeted vs Untargeted: Concepts & Design Session 2 — Standards, Calibration Curves & QC Layout Session 3 — Targeted Acquisition & Quantitation Workflow Session 4 — Mini Capstone: Assay Development & Validation Plan

Session 1

Fee: Rs 8800

Targeted vs Untargeted: Concepts & Design

Role of targeted metabolomics in research and clinics

hypothesis driven panels biomarker verification & validation fit for purpose assay design

Selecting metabolites, matrices and dynamic ranges

panel definition and inclusion criteria serum / plasma / urine / tissue expected concentration ranges

Workflow overview from sample to report

sample prep and extraction LC/GC–MS method and transitions quantitation and QC review

Session 2

Fee: Rs 11800

Standards, Calibration Curves & QC Layout

Reference standards and internal standards

neat standards and mixtures stable isotope labelled IS spiking strategies and timing

Preparing calibration and QC samples

stock and working solutions calibration levels and ranges low / mid / high QCs

Calibration models, LOD/LOQ and acceptance rules

linear / weighted regression back calculated accuracy LOD/LOQ concepts & criteria

Session 3

Fee: Rs 14800

Targeted Acquisition & Quantitation Workflow

Targeted MS modes and method parameters

MRM / SRM / PRM / SIM transitions, collision energy dwell time and scheduling

Peak integration and quantitation rules

peak shape and manual review area ratios to internal standards handling interferences and flags

QC monitoring and batch level checks

QC accuracy and precision plots carryover and stability checks run acceptance/rejection criteria

Session 4

Fee: Rs 18800

Mini Capstone: Assay Development & Validation Plan

Designing a targeted metabolomics assay for a use case

clinical, nutrition or toxicology study

Validation parameters and acceptance criteria

linearity and range precision, accuracy and recovery matrix effects and stability tests

Deliverables: assay specification, validation checklist & report template

panel & matrix specification sheet validation plan/checklist result table & narrative template

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Targeted Metabolomics — Standards & Quantitation Training | Panels, Calibration & QC

Targeted Metabolomics — Standards & Quantitation — Hands-on