Targeted Proteomics — SRM, PRM and MRM — Hands-on

Learn how to design, optimize and interpret targeted proteomics assays using SRM, PRM and MRM. This module focuses on transition selection, method setup, calibration and QC concepts that support sensitive and precise quantitation of predefined protein and peptide panels for validation and routine applications.

Targeted Proteomics — SRM, PRM and MRM

Help Desk · WhatsApp

Session Index

Session 1 — Targeted Proteomics Concepts & Assay Types Session 2 — SRM/MRM Method Design & Optimization Session 3 — PRM & High-Resolution Targeted Workflows Session 4 — Mini Capstone: Targeted Panel Design & Reporting

Session 1

Fee: Rs 8800

Targeted Proteomics Concepts & Assay Types

Why targeted proteomics vs discovery workflows

hypothesis driven quantitation biomarker verification assay robustness and transfer

SRM, PRM and MRM assay families (conceptual view)

triple quadrupole SRM/MRM Orbitrap or TOF based PRM similarities and differences

Use cases and panel types for targeted proteomics

biomarker panels pathway or signature panels QC and system suitability panels

Session 2

Fee: Rs 11800

SRM/MRM Method Design & Optimization (Concepts)

Peptide and transition selection principles

proteotypic peptide concepts interference awareness number of transitions per peptide

Scheduling and dwell time thinking for SRM/MRM

retention time windows cycle time concepts balancing multiplexing with data quality

Calibration curves, LOD/LOQ and linearity (theory level)

spike in standards idea dynamic range concepts basic acceptance criteria thinking

Session 3

Fee: Rs 14800

PRM & High-Resolution Targeted Workflows (Concepts)

PRM basics and comparison with SRM/MRM

isolation of precursor then full MS/MS high resolution fragment readout flexibility for new transitions post acquisition

Method structure and scheduling for PRM assays

inclusion lists concepts isolation window choices cycle time and number of targets

Data extraction and chromatogram review at concept level

XIC and peak integration ideas transition ratio checks basic QC plots for targeted data

Session 4

Fee: Rs 18800

Mini Capstone: Targeted Panel Design & Reporting

Design exercise for a small targeted proteomics panel

Theory + Practical (planning workflow)

QC and reporting structure for targeted assays (concept level)

precision and accuracy summaries CVs, blanks and standards overview basic report tables and plots

Deliverables: panel design sheet, method outline and QC checklist

target and peptide list assay concept summary QC and acceptance criteria outline

PDF

Targeted Proteomics Training | SRM, PRM & MRM Assay Design and Quantitation

Targeted Proteomics — SRM, PRM and MRM — Hands-on