Taxonomic Profiling — ASVs, OTUs & Classifiers — Hands-on

Learn how to convert cleaned amplicon or shotgun reads into reliable taxonomic profiles. You will understand ASV vs OTU workflows, choose and curate reference databases, configure classifiers, and compute alpha or beta diversity with figures suitable for manuscripts and reports.

Taxonomic Profiling — ASVs, OTUs & Classifiers

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Session Index

Session 1 — ASVs, OTUs & Amplicon Inference Session 2 — Reference Databases & Classifiers Session 3 — Diversity Metrics & Community Structure Session 4 — Mini Capstone: Taxonomic Profiles to Figures

Session 1

Fee: Rs 8800

ASVs, OTUs & Amplicon Inference

ASV vs OTU concepts and implications

denoised sequence variants clustering based OTUs resolution vs robustness

Amplicon inference workflows end to end

error models and denoising chimera removal concepts paired read merging logic

Feature tables and sequence objects

feature count matrices representative sequences export formats for tools

Session 2

Fee: Rs 11800

Reference Databases & Classifiers

Curation of reference databases and taxonomies

SILVA and RDP style resources domain specific reference sets versioning and provenance

Classifier models and parameter choices

naive Bayes style classifiers alignment based assignment confidence thresholds and cutoffs

Validation with mocks and benchmarks

mock community evaluation precision and recall thinking common misclassification patterns

Session 3

Fee: Rs 14800

Diversity Metrics & Community Structure

Alpha diversity indices and rarefaction mindset

richness and Shannon indices Simpson and evenness metrics rarefaction and coverage thinking

Beta diversity and ordination plots

Bray Curtis and Jaccard UniFrac style distances PCoA and NMDS visualizations

Community summaries for stakeholders

stacked bar and heat maps core microbiome summaries export ready tables and figures

Session 4

Fee: Rs 18800

Mini Capstone: Taxonomic Profiles to Figures

From feature table to publication style plots

Theory plus hands on

Reporting taxonomic results with metadata context

group comparisons and contrasts effect sizes and uncertainty narratives for collaborators

Deliverables: profiles, plots and methods block

exported abundance tables figure panels and legends methods and parameter appendix

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Taxonomic Profiling ASVs OTUs & Classifiers Training | Diversity Metrics & Publication Plots

Taxonomic Profiling — ASVs, OTUs & Classifiers — Hands-on