TCR & BCR Repertoire Analysis — Hands-on

Develop an intuitive, analysis ready view of T cell and B cell receptor repertoires. This module covers biological foundations, core repertoire data structures, clonotype definitions, diversity metrics and conceptual workflows for tracking clonal dynamics across time, tissue and clinical groups.

TCR & BCR Repertoire Analysis

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Session Index

Session 1 — Repertoire Biology & Sequencing Overview Session 2 — Clonotypes, CDR3 & Diversity Metrics Session 3 — Comparing Repertoires & Clonal Dynamics Session 4 — Mini Project: Repertoire Storyboard

Session 1

Fee: Rs 8800

Repertoire Biology & Sequencing Overview

TCR and BCR biology and why repertoires matter

V (D) J recombination concepts clonal selection and expansion links to infection, vaccination and cancer

Sample types and repertoire sequencing strategies (conceptual)

peripheral blood, tissue and tumour samples bulk versus single cell oriented view alpha beta TCR, gamma delta TCR and BCR heavy light chains

High level repertoire data outputs and file structures (orientation)

annotated rearrangements table concept V, D, J calls and CDR3 sequences counts, frequencies and metadata columns

Session 2

Fee: Rs 11800

Clonotypes, CDR3 & Diversity Metrics

Defining clonotypes from rearrangements (conceptual options)

CDR3 sequence based grouping V and J gene usage considerations nucleotide versus amino acid level definitions

Basic repertoire summaries and diversity metrics (orientation)

richness and clonality ideas Shannon and Simpson style indices (conceptual) Gini and related inequality views

CDR3 length, composition and motif oriented summaries

length distributions and usage plots amino acid property based views motif level orientation for public specificities

Session 3

Fee: Rs 14800

Comparing Repertoires & Clonal Dynamics

Concepts for comparing repertoires across samples or groups

overlap and shared clonotypes distance based comparisons (orientation) public versus private repertoire concepts

Tracking clonal expansion and contraction over time (conceptual)

following selected clonotypes across visits link to vaccination or treatment time points visual motifs for expansion trajectories

Contextualising patterns in infection, vaccination and oncology examples

acute versus chronic infection patterns vaccine responders versus non responders (conceptual) tumour infiltrating lymphocyte repertoires (orientation)

Session 4

Fee: Rs 18800

Mini Project: Repertoire Storyboard

From annotated repertoire table to core plots and summaries (conceptual workflow)

diversity and clonality overview CDR3 length and VJ usage style plots top clonotypes table with simple annotations

Building a simple clonal dynamics storyboard (conceptual)

selecting clonotypes of interest depicting change across time or conditions relating patterns to clinical or experimental events

Summarising findings for immunology and translational teams

short text narrative with key figures tables and plots suitable for slide decks handoff to neoantigen, biomarker and trial modules

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TCR & BCR Repertoire Analysis Training | Clonotypes, Diversity & Immune Dynamics

TCR & BCR Repertoire Analysis — Hands-on