Viruses & Phages — Virome & Phageome Inference — Hands-on

Learn how to move from generic metagenomes to focused views of viruses and phages. This module covers virome study design, viral read handling, assembly and viral contig identification, quality and taxonomy assessment, basic host linking strategies and ecological interpretation of virome and phageome profiles.

Viruses & Phages — Virome & Phageome Inference

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Session Index

Session 1 — Virome & Phageome Concepts & Study Design Session 2 — Viral Read Processing, Assembly & Identification Session 3 — Viral Genome QC, Taxonomy & Host Linking Session 4 — Mini Capstone: Virome/Phageome Report

Session 1

Fee: Rs 8800

Virome & Phageome Concepts & Study Design

Bacteriophages, viruses and the microbiome

lytic vs temperate phages prophages and viral dark matter roles in community structure and AMR

Virome and phageome study designs

enriched viromes vs total metagenomes sample types (gut, wastewater, soil, marine) longitudinal and intervention scenarios

Wet lab and sequencing considerations (concepts only)

viral particle enrichment overview host DNA background read length and platform choices

Session 2

Fee: Rs 11800

Viral Read Processing, Assembly & Identification

QC and host removal for virome data

FastQC / MultiQC review trimming adapters and low quality bases mapping to host genomes for depletion

Assemblies tuned for viral contigs

short read assemblers overview contig length and coverage considerations co assembly vs per sample assembly ideas

Viral contig identification concepts

k mer and gene content based tools overview separating viral, bacterial and plasmid contigs basic thresholds and score interpretation

Session 3

Fee: Rs 14800

Viral Genome QC, Taxonomy & Host Linking

Quality assessment of viral contigs and genomes

completeness and contamination ideas length and circularity checks flagging low confidence elements

Assigning viral taxonomy and clustering

taxonomy frameworks for viruses overview clustering into viral populations building non redundant viral catalogs

Linking viruses and phages to hosts

CRISPR spacer matches and k mer similarity concepts co abundance based hints summarising virus host networks at high level

Session 4

Fee: Rs 18800

Mini Capstone: Virome/Phageome Report

Designing a small virome or phageome analysis

guided theory plus practical

From viral contigs to ecological and functional summaries

viral abundance and diversity tables simple virus host or phage host views figures linking viruses to environment or phenotype

Deliverables: virome summary figures, tables and short narrative

barplots and heatmaps for viral groups catalog or feature table exports brief report suitable for methods or results sections

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Viruses & Phages — Virome & Phageome Inference Training | Viral Metagenomics & Phage Ecology

Viruses & Phages — Virome & Phageome Inference — Hands-on