Fee: Rs 2,12,400/-

Protocols List

1. Cell Culture Techniques: Aseptic Technique for Culture Maintenance, CO2 Incubator Setup and Validation, Primary Cell Isolation and Culture from Tissues, Cell Line Authentication via STR Profiling, Cryopreservation and Thawing Protocols for Cell Lines, Serum-free Culture for Bioproduction, Hypoxic Conditions Setup for Cancer Cell Studies, 3D Cell Culture Techniques including Spheroids and Organoids, Automated Cell Culture Systems for High-throughput Screening
2. Flow Cytometry for Cell Sorting: Single-Cell Sorting for Clonal Analysis, Multiparametric Sorting using 8+ Color Panels, High-Speed Cell Sorting for Large Sample Volumes, Sterile Sorting for Direct Culture and Transplantation, Intracellular Cytokine Staining and Sorting, Apoptosis and Cell Cycle Analysis via Flow Cytometry, Fluorescent Protein Sorting (e.g., GFP, RFP) , Rare Population Sorting with High Sensitivity Detectors, Use of Sorting Chips for Microfluidic Flow Cytometry
3. Cellular Imaging with Confocal Microscopy: Live Cell Imaging for Dynamic Process Observation, FRET (Fluorescence Resonance Energy Transfer) for Molecular Interactions, FRAP (Fluorescence Recovery After Photobleaching) to Measure Protein Mobility, Super-resolution Techniques beyond Diffraction Limit, Multi-photon Excitation for Deep Tissue Imaging, Time-lapse Confocal Microscopy for Cellular Behavior, Z-stacking and 3D Reconstruction from Confocal Slices, Use of Fluorescent Dyes and Probes Specific to Organelles, Quantitative Image Analysis for Cellular Metrics

Fee: Rs 1,71,100/-

Protocols List

1. ELISA Enhancements: High-throughput robotic ELISA platforms, Multiplex ELISA for simultaneous detection of multiple targets, Digital ELISA for ultra-sensitive detection using single-molecule counting, Bead-based ELISA for enhanced capture efficiency, Time-resolved fluorescence ELISA for improved signal stability
2. Antigen Preparation Innovations: Cell-free expression systems for rapid protein synthesis, Use of molecular chaperones for proper protein folding, Transient expression in mammalian cells for post-translational modifications, Use of immobilized enzymes for tag cleavage, High-pressure refolding techniques for disulfide-rich proteins
3. Advanced Antigen Immunization Strategies: Use of nanoparticle-based adjuvants for enhanced immune response, Intralymphatic immunization for direct lymph node delivery, Use of gene electrotransfer for in vivo antigen expression, Implementing oral immunization strategies with encapsulated antigens, Autoantigen-based tolerization techniques for therapeutic antibody development
4. Sophisticated Antibody Titre Analysis: Utilization of electrochemiluminescence for sensitive titre measurements, Biacore surface plasmon resonance for affinity and concentration, High-content screening for functional antibodies, Single B cell screening for rapid clone identification, Immuno-PCR for combining PCR sensitivity with antibody specificity
5. Comprehensive Bleeding Techniques: Use of automated decapicone for high-throughput animal bleeding, Laser Doppler for real-time blood flow measurement during bleeding, Application of humane endpoints for ethical bleeding, Spectroscopic techniques for blood component analysis, Integration of stress-free handling equipment to minimize animal distress during bleeding
6. Plasma Pheresis Advanced Protocols: Use of closed-system automated apheresis to prevent contamination, Application of differential centrifugation for selective plasma component retrieval, High-efficiency membrane separators for improved plasma yield, Real-time monitoring of plasma extraction parameters, Use of anticoagulant recycling systems to reduce waste
7. Antibody Extraction from Plasma: Supercritical fluid extraction for antibody isolation, Partitioning in aqueous two-phase systems, Affinity partitioning using smart polymers, Continuous extraction systems for scale-up, Phase inversion precipitation for selective antibody isolation
8. Highly Specialized Antibody Purification: Use of immunoaffinity columns specific to subclass or species, Sequential multi-step chromatography for removing aggregates, Utilization of fluidized bed chromatography for gentle processing, Cryogenic purification techniques for temperature-sensitive antibodies, Dynamic light scattering for real-time purity assessment
9. In-depth Antibody Purity Analysis: Application of 2D-DIGE for differential analysis, MALDI-TOF mass spectrometry for molecular weight determination, Capillary electrophoresis for charge heterogeneity, Use of chromatofocusing for isoelectric point determination, Automated Western blot for high-throughput screening
10. Coomassie Blue Staining Techniques: Use of reversible zinc staining for downstream MS analysis, Application of pre-stain protein loading controls for quantitative analysis, Implementation of gel documentation systems with fluorescence detection, Dual staining with imidazole-zinc and Coomassie for differential visualization, Use of eco-friendly Coomassie brilliant blue formulations for reduced toxicity
11. Silver Staining Protocols: Use of formaldehyde-free silver staining for reduced toxicity, Silver enhancement for nanoparticle visualization in tissues, Microwave-assisted silver staining for rapid processing, Use of alternative reducing agents for enhanced clarity, Multi-metal staining techniques for differential protein visualization

Fee: Rs 1,41,600/-

Protocols List

1. RNA Extraction from Tissue: Homogenization in Lysis Buffer, Use of Silica Columns or Magnetic Beads for RNA Purification, DNase Treatment to Remove DNA Contamination, RNA Integrity Assessment via Gel Electrophoresis or Bioanalyzer
2. Reverse Transcriptase-PCR: Primer Design for Target RNA, Reverse Transcription of RNA to cDNA Using Reverse Transcriptase, PCR Amplification of cDNA, Gel Electrophoresis for PCR Product Verification
3. Vector Design: Selection of Cloning or Expression Vector, Multiple Cloning Site (MCS) Analysis, Incorporation of Selectable Markers and Reporter Genes, Optimization of Regulatory Sequences for Expression
4. Isolation of DNA: Cell Lysis and DNA Extraction from Various Tissue Sources, Phenol-Chloroform Extraction or Silica-Based DNA Purification, Quantification and Qualitative Assessment via Spectrophotometry or Fluorometry
5. Vector Selection: Plasmid, Bacterial Artificial Chromosome (BAC) , or Yeast Artificial Chromosome (YAC) Selection Based on Application, Comparison of Copy Number and Stability, Antibiotic Resistance Marker Consideration
6. Restriction Digest: Selection of Restriction Enzymes Compatible with Vector and Insert, Setup of Digestion Reactions, Analysis of Digestion Efficiency via Agarose Gel Electrophoresis, Purification of Digested Products
7. Ligation: Preparation of Vector and Insert at Optimal Ratios, Use of T4 DNA Ligase for Covalent Bond Formation, Control Reactions Without Insert to Check Background, Transformation Efficiency Test of Ligation Mixture
8. Transformation: Preparation of Competent Cells (Chemical or Electroporation Competency) , Heat Shock or Electroporation of Cells with Ligation Mixture, Recovery in SOC or LB Media, Plating on Selective Media
9. Blue White Screening: Use of X-gal Plates to Distinguish Between Recombinant and Non-Recombinant Clones, IPTG Induction of LacZ Marker, Selection of White Colonies for Further Analysis
10. Colony Picking: Sterile Technique for Isolating Individual Colonies, Inoculation in Liquid Culture for Amplification, Storage of Clones in Glycerol Stocks at -80°C
11. Plasmid Extraction: Small-Scale (Miniprep) or Large-Scale (Maxiprep) Plasmid DNA Isolation, Plasmid DNA Purification Using Spin Columns or Anion Exchange Chromatography, Determination of Plasmid Concentration and Purity
12. Sequencing: Preparation of DNA for Sanger Sequencing or Next-Generation Sequencing, Primer Design for Specific Regions, Submission of Samples to Sequencing Facility, Analysis of Sequencing Data for Verification of Cloning

Fee: Rs 2,71,400/-

Protocols List

1. Culture Media Preparation: Autoclaving Media, pH Adjustment, Sterile Filtration Techniques
2. Isolation Techniques: Serial Dilution for Plating, Single Colony Isolation using a Micromanipulator, Automated Colony Pickers
3. Biochemical Testing: Automated Microbial Identification Systems (VITEK, MALDI-TOF) , Multi-Test Biochemical Panels, Enzyme Activity Assays
4. Molecular Identification: Real-Time PCR for Rapid Identification, High-Throughput Sequencing for Community Profiling, Multiplex PCR for Pathogen Detection
5. Whole Genome Sequencing: Library Preparation for Illumina, SMRTbell Library Construction for PacBio, Direct RNA Sequencing for Real-Time Applications
6. Site-Directed Mutagenesis Protocols: Temperature Gradient PCR for Mutation Screening, Use of Modified Nucleotides for Error-Prone PCR, High-Fidelity Enzymes for Minimal Errors
7. Overlap Extension PCR: Optimization of Primer Concentrations for Maximum Yield, Use of Thermostable DNA Polymerases, Adjustment of Annealing Temperatures for Different GC Contents
8. Commercial Kits for Mutagenesis: High-Efficiency Cloning Vectors, Seamless Cloning using Gibson Assembly, Use of Homologous Recombination Kits
9. Mega-primer Method: Primer Design for Target-Specific Mutagenesis, Use of Asymmetric PCR for Primer Amplification, Purification Techniques for Mega Primers
10. Double-Stranded Plasmid Mutagenesis: Use of Methyl-Sensitive Restriction Enzymes, Cold-Shock Expression Systems for Plasmid Stabilization, Post-Transformation Cure of Template DNA
11. Lambda Red Recombination: Induction of Lambda Prophage for Increased Efficiency, Recombineering with Linear DNA Fragments, Electrocompetent Cell Protocols Specific to Recombineering
12. Bacterial Adaptive Mutagenesis: Use of Reporters for Mutation Detection, Automated Systems for Mutation Rate Analysis, Environmental Stress Chambers for Induced Mutagenesis
13. CRISPR/Cas9 Techniques: Dual gRNA Design for Large Deletions, TracrRNA and crRNA Custom Synthesis, Lipid Nanoparticle Delivery Systems for In Vivo Editing
14. Competent Cell Preparation: RbCl2 Preparation Method, Use of Different Strains for Optimized Uptake (DH5 alpha, BL21) , Protocol Variations for Ultra-Competent Cells
15. DNA Transformation Methods: Microinjection for Direct Nucleus Delivery, Bioballistics for Plant Transformation, Silicon Carbide Whiskers for Transformation
16. Electroporation Settings: Optimization of Capacitance for Different Cell Types, Use of Multi-Pulse Electroporation for Increased Efficiency, Software-Controlled Electroporation Parameters
17. Antibiotic Selection Markers: Use of Dual Antibiotic Selection for Stability, Inducible Promoters for Controlled Expression, Linker Peptides for Cleavable Fusion Protein
18. Screening Techniques: Automated Fluorescence-Activated Cell Sorting (FACS) for High-Throughput Screening, Digital Droplet PCR for Precise Quantification, Chemiluminescent Reporters for Sensitive Detection
19. Bioreactor Operation: Software-Controlled pH and Dissolved Oxygen Probes, Use of Optical Density Sensors for Growth Monitoring, Automated Feeding Systems for Optimized Growth
20. GC-MS for Metabolite Profiling: Derivatization Techniques for Improved Volatility, Time-of-Flight (TOF) Detectors for Rapid Analysis, Tandem MS for Structural Elucidation
21. Fermentation Optimization: Use of Oscillating Perfusion Systems for Improved Cell Density, Dynamic Nutrient Feeding Algorithms, Real-Time Metabolite Monitoring Systems
22. Pilot Scale Production: CIP (Clean-In-Place) Systems for Bioreactors, Scale-Up Simulation Software, Inline Purification Technologies
23. Quality Control Testing: Real-Time Stability Testing under Accelerated Conditions, Use of Biosensors for Contaminant Detection, High-Throughput Sequencing for Genetic Stability Testing
24. Regulatory Compliance Documentation: Electronic Lab Notebooks for Data Integrity, Use of Regulatory Information Management Systems, Preparation of Submission-Ready Documentation Packages

Fee: Rs 2,71,400/-

Protocols List

1. Genetic Modification of Crops: Vector Construction for Gene Insertion, Electroporation of Plant Cells, Floral Dip Method for Agrobacterium Transformation, Biolistic Particle Delivery System Setup, Selectable Marker Gene Incorporation, Screening for Transgenic Plants, Gene Editing via CRISPR-Cas9: Guide RNA Design, Cas9 Protein Delivery via Plasmid or Ribonucleoprotein, TALENs: Target Sequence Identification, Assembly of Custom TALENs, Zinc Finger Nucleases (ZFNs) : Zinc Finger Protein Design, mRNA Synthesis for ZFN Delivery, Field Trial Setup for GM Crops, Regulatory Compliance for GMO Release
2. Soil Microbiome Analysis: Soil Sampling and Homogenization, DNA Extraction Using Commercial Kits, PCR Amplification of 16S rRNA Genes, Library Preparation for Sequencing, High-Throughput Sequencing on Illumina or PacBio, Bioinformatics: OTU Clustering and Taxonomic Assignment, Metagenomic Shotgun Library Preparation, Sequencing Data Analysis: Gene Prediction and Functional Annotation, RNA Extraction and Reverse Transcription for RNA-Seq, cDNA Library Construction and Sequencing for Metatranscriptomics, Data Integration and Microbial Community Interpretation, Stable Isotope Probing: DNA and RNA SIP, Analysis of Labeled DNA/RNA to Trace Metabolic Pathways
3. Plant Tissue Culture Techniques: Preparation of Nutrient Media Specific to Plant Species, Autoclaving Media and Culture Vessels, Surface Sterilization of Explants Using Chemical Agents, Establishment of Aseptic Culture Conditions, Callus Induction Using Plant Hormones (Auxins and Cytokinins) , Subculturing Callus onto Regeneration Media, Inducing Shoot and Root Formation via Hormonal Manipulation, Micropropagation Through Meristem Culture, Somatic Embryo Development and Germination, Hardening Techniques for Tissue Cultured Plants: Gradual Adaptation to External Environment, Acclimatization in Greenhouse Conditions, Quality Assessment of Clonal Fidelity, Genetic Stability Testing Using Molecular Markers

1. Aseptic Technique for Culture Maintenance | Fee: Rs 9,440.00 ( Rupees Nine Thousand Four Hundred And Forty )
2. CO2 Incubator Setup and Validation | Fee: Rs 35,400.00 ( Rupees Thirty Five Thousand Four Hundred )
3. Primary Cell Isolation and Culture from Tissues | Fee: Rs 35,400.00 ( Rupees Thirty Five Thousand Four Hundred )
4. Cell Line Authentication via STR Profiling | Fee: Rs 88,500.00 ( Rupees Eighty Eight Thousand Five Hundred )
5. Cryopreservation and Thawing Protocols for Cell Lines | Fee: Rs 17,700.00 ( Rupees Seventeen Thousand Seven Hundred )
6. Serum-free Culture for Bioproduction | Fee: Rs 64,900.00 ( Rupees Sixty Four Thousand Nine Hundred )
7. Hypoxic Conditions Setup for Cancer Cell Studies | Fee: Rs 147,500.00 ( Rupees One Lakh Forty Seven Thousand Five Hundred )
8. 3D Cell Culture Techniques including Spheroids and Organoids | Fee: Rs 354,000.00 ( Rupees Three Lakh Fifty Four Thousand )
9. Automated Cell Culture Systems for High-throughput Screening | Fee: Rs 354,000.00 ( Rupees Three Lakh Fifty Four Thousand )
10. Single-Cell Sorting for Clonal Analysis | Fee: Rs 354,000.00 ( Rupees Three Lakh Fifty Four Thousand )
11. Multiparametric Sorting using 8+ Color Panels | Fee: Rs 354,000.00 ( Rupees Three Lakh Fifty Four Thousand )
12. High-Speed Cell Sorting for Large Sample Volumes | Fee: Rs 354,000.00 ( Rupees Three Lakh Fifty Four Thousand )
13. Sterile Sorting for Direct Culture and Transplantation | Fee: Rs 354,000.00 ( Rupees Three Lakh Fifty Four Thousand )
14. Intracellular Cytokine Staining and Sorting | Fee: Rs 129,800.00 ( Rupees One Lakh Twenty Nine Thousand Eight Hundred )
15. Apoptosis and Cell Cycle Analysis via Flow Cytometry | Fee: Rs 354,000.00 ( Rupees Three Lakh Fifty Four Thousand )
16. Fluorescent Protein Sorting (e.g., GFP, RFP) | Fee: Rs 236,000.00 ( Rupees Two Lakh Thirty Six Thousand )
17. Rare Population Sorting with High Sensitivity Detectors | Fee: Rs 236,000.00 ( Rupees Two Lakh Thirty Six Thousand )
18. Use of Sorting Chips for Microfluidic Flow Cytometry | Fee: Rs 236,000.00 ( Rupees Two Lakh Thirty Six Thousand )
19. Live Cell Imaging for Dynamic Process Observation | Fee: Rs 354,000.00 ( Rupees Three Lakh Fifty Four Thousand )
20. FRET (Fluorescence Resonance Energy Transfer) for Molecular Interactions | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
21. FRAP (Fluorescence Recovery After Photobleaching) to Measure Protein Mobility | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
22. Super-resolution Techniques beyond Diffraction Limit | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
23. Multi-photon Excitation for Deep Tissue Imaging | Fee: Rs 472,000.00 ( Rupees Four Lakh Seventy Two Thousand )
24. Time-lapse Confocal Microscopy for Cellular Behavior | Fee: Rs 472,000.00 ( Rupees Four Lakh Seventy Two Thousand )
25. Z-stacking and 3D Reconstruction from Confocal Slices | Fee: Rs 472,000.00 ( Rupees Four Lakh Seventy Two Thousand )
26. Use of Fluorescent Dyes and Probes Specific to Organelles | Fee: Rs 472,000.00 ( Rupees Four Lakh Seventy Two Thousand )
27. Quantitative Image Analysis for Cellular Metrics | Fee: Rs 472,000.00 ( Rupees Four Lakh Seventy Two Thousand )
28. High-throughput robotic ELISA platforms | Fee: Rs 472,000.00 ( Rupees Four Lakh Seventy Two Thousand )
29. Multiplex ELISA for simultaneous detection of multiple targets | Fee: Rs 100,300.00 ( Rupees One Lakh Three Hundred )
30. Digital ELISA for ultra-sensitive detection using single-molecule counting | Fee: Rs 177,000.00 ( Rupees One Lakh Seventy Seven Thousand )
31. Bead-based ELISA for enhanced capture efficiency | Fee: Rs 177,000.00 ( Rupees One Lakh Seventy Seven Thousand )
32. Time-resolved fluorescence ELISA for improved signal stability | Fee: Rs 177,000.00 ( Rupees One Lakh Seventy Seven Thousand )
33. Cell-free expression systems for rapid protein synthesis | Fee: Rs 177,000.00 ( Rupees One Lakh Seventy Seven Thousand )
34. Use of molecular chaperones for proper protein folding | Fee: Rs 295,000.00 ( Rupees Two Lakh Ninety Five Thousand )
35. Transient expression in mammalian cells for post-translational modifications | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
36. Use of immobilized enzymes for tag cleavage | Fee: Rs 177,000.00 ( Rupees One Lakh Seventy Seven Thousand )
37. High-pressure refolding techniques for disulfide-rich proteins | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
38. Use of nanoparticle-based adjuvants for enhanced immune response | Fee: Rs 295,000.00 ( Rupees Two Lakh Ninety Five Thousand )
39. Intralymphatic immunization for direct lymph node delivery | Fee: Rs 354,000.00 ( Rupees Three Lakh Fifty Four Thousand )
40. Use of gene electrotransfer for in vivo antigen expression | Fee: Rs 295,000.00 ( Rupees Two Lakh Ninety Five Thousand )
41. Implementing oral immunization strategies with encapsulated antigens | Fee: Rs 295,000.00 ( Rupees Two Lakh Ninety Five Thousand )
42. Autoantigen-based tolerization techniques for therapeutic antibody development | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
43. Utilization of electrochemiluminescence for sensitive titre measurements | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
44. Biacore surface plasmon resonance for affinity and concentration | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
45. High-content screening for functional antibodies | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
46. Single B cell screening for rapid clone identification | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
47. Immuno-PCR for combining PCR sensitivity with antibody specificity | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
48. Use of automated decapicone for high-throughput animal bleeding | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
49. Laser Doppler for real-time blood flow measurement during bleeding | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
50. Application of humane endpoints for ethical bleeding | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
51. Spectroscopic techniques for blood component analysis | Fee: Rs 177,000.00 ( Rupees One Lakh Seventy Seven Thousand )
52. Integration of stress-free handling equipment to minimize animal distress during bleeding | Fee: Rs 295,000.00 ( Rupees Two Lakh Ninety Five Thousand )
53. Use of closed-system automated apheresis to prevent contamination | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
54. Application of differential centrifugation for selective plasma component retrieval | Fee: Rs 295,000.00 ( Rupees Two Lakh Ninety Five Thousand )
55. High-efficiency membrane separators for improved plasma yield | Fee: Rs 295,000.00 ( Rupees Two Lakh Ninety Five Thousand )
56. Real-time monitoring of plasma extraction parameters | Fee: Rs 295,000.00 ( Rupees Two Lakh Ninety Five Thousand )
57. Use of anticoagulant recycling systems to reduce waste | Fee: Rs 295,000.00 ( Rupees Two Lakh Ninety Five Thousand )
58. Supercritical fluid extraction for antibody isolation | Fee: Rs 295,000.00 ( Rupees Two Lakh Ninety Five Thousand )
59. Partitioning in aqueous two-phase systems | Fee: Rs 295,000.00 ( Rupees Two Lakh Ninety Five Thousand )
60. Affinity partitioning using smart polymers | Fee: Rs 295,000.00 ( Rupees Two Lakh Ninety Five Thousand )
61. Continuous extraction systems for scale-up | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
62. Phase inversion precipitation for selective antibody isolation | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
63. Use of immunoaffinity columns specific to subclass or species | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
64. Sequential multi-step chromatography for removing aggregates | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
65. Utilization of fluidized bed chromatography for gentle processing | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
66. Cryogenic purification techniques for temperature-sensitive antibodies | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
67. Dynamic light scattering for real-time purity assessment | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
68. Application of 2D-DIGE for differential analysis | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
69. MALDI-TOF mass spectrometry for molecular weight determination | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
70. Capillary electrophoresis for charge heterogeneity | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
71. Use of chromatofocusing for isoelectric point determination | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
72. Automated Western blot for high-throughput screening | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
73. Use of reversible zinc staining for downstream MS analysis | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
74. Application of pre-stain protein loading controls for quantitative analysis | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
75. Implementation of gel documentation systems with fluorescence detection | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
76. Dual staining with imidazole-zinc and Coomassie for differential visualization | Fee: Rs 413,000.00 ( Rupees Four Lakh Thirteen Thousand )
77. Use of eco-friendly Coomassie brilliant blue formulations for reduced toxicity | Fee: Rs 100,300.00 ( Rupees One Lakh Three Hundred )
78. Use of formaldehyde-free silver staining for reduced toxicity | Fee: Rs 1,003,000.00 ( Rupees Ten Lakh Three Thousand )
79. Silver enhancement for nanoparticle visualization in tissues | Fee: Rs 295,000.00 ( Rupees Two Lakh Ninety Five Thousand )
80. Microwave-assisted silver staining for rapid processing | Fee: Rs 177,000.00 ( Rupees One Lakh Seventy Seven Thousand )
81. Use of alternative reducing agents for enhanced clarity | Fee: Rs 112,100.00 ( Rupees One Lakh Twelve Thousand One Hundred )
82. Multi-metal staining techniques for differential protein visualization | Fee: Rs 177,000.00 ( Rupees One Lakh Seventy Seven Thousand )
83. Homogenization in Lysis Buffer | Fee: Rs 38,940.00 ( Rupees Thirty Eight Thousand Nine Hundred And Forty )
84. Use of Silica Columns or Magnetic Beads for RNA Purification | Fee: Rs 47,200.00 ( Rupees Forty Seven Thousand Two Hundred )
85. DNase Treatment to Remove DNA Contamination | Fee: Rs 4,720.00 ( Rupees Four Thousand Seven Hundred And Twenty )
86. RNA Integrity Assessment via Gel Electrophoresis or Bioanalyzer | Fee: Rs 5,900.00 ( Rupees Five Thousand Nine Hundred )
87. Primer Design for Target RNA | Fee: Rs 7,080.00 ( Rupees Seven Thousand Eighty )
88. Reverse Transcription of RNA to cDNA Using Reverse Transcriptase | Fee: Rs 9,440.00 ( Rupees Nine Thousand Four Hundred And Forty )
89. PCR Amplification of cDNA | Fee: Rs 5,900.00 ( Rupees Five Thousand Nine Hundred )
90. Gel Electrophoresis for PCR Product Verification | Fee: Rs 3,540.00 ( Rupees Three Thousand Five Hundred And Forty )
91. Selection of Cloning or Expression Vector | Fee: Rs 9,440.00 ( Rupees Nine Thousand Four Hundred And Forty )
92. Multiple Cloning Site (MCS) Analysis | Fee: Rs 9,440.00 ( Rupees Nine Thousand Four Hundred And Forty )
93. Incorporation of Selectable Markers and Reporter Genes | Fee: Rs 41,300.00 ( Rupees Forty One Thousand Three Hundred )
94. Optimization of Regulatory Sequences for Expression | Fee: Rs 41,300.00 ( Rupees Forty One Thousand Three Hundred )
95. Cell Lysis and DNA Extraction from Various Tissue Sources | Fee: Rs 7,080.00 ( Rupees Seven Thousand Eighty )
96. Phenol-Chloroform Extraction or Silica-Based DNA Purification | Fee: Rs 7,080.00 ( Rupees Seven Thousand Eighty )
97. Quantification and Qualitative Assessment via Spectrophotometry or Fluorometry | Fee: Rs 4,720.00 ( Rupees Four Thousand Seven Hundred And Twenty )
98. Plasmid, Bacterial Artificial Chromosome (BAC) , or Yeast Artificial Chromosome (YAC) Selection Based on Application | Fee: Rs 7,080.00 ( Rupees Seven Thousand Eighty )
99. Comparison of Copy Number and Stability | Fee: Rs 7,080.00 ( Rupees Seven Thousand Eighty )
100. Antibiotic Resistance Marker Consideration | Fee: Rs 17,700.00 ( Rupees Seventeen Thousand Seven Hundred )
101. Selection of Restriction Enzymes Compatible with Vector and Insert | Fee: Rs 10,620.00 ( Rupees Ten Thousand Six Hundred And Twenty )
102. Setup of Digestion Reactions | Fee: Rs 4,720.00 ( Rupees Four Thousand Seven Hundred And Twenty )
103. Analysis of Digestion Efficiency via Agarose Gel Electrophoresis | Fee: Rs 5,900.00 ( Rupees Five Thousand Nine Hundred )
104. Purification of Digested Products | Fee: Rs 7,080.00 ( Rupees Seven Thousand Eighty )
105. Preparation of Vector and Insert at Optimal Ratios | Fee: Rs 14,160.00 ( Rupees Fourteen Thousand One Hundred And Sixty )
106. Use of T4 DNA Ligase for Covalent Bond Formation | Fee: Rs 7,080.00 ( Rupees Seven Thousand Eighty )
107. Control Reactions Without Insert to Check Background | Fee: Rs 9,440.00 ( Rupees Nine Thousand Four Hundred And Forty )
108. Transformation Efficiency Test of Ligation Mixture | Fee: Rs 14,160.00 ( Rupees Fourteen Thousand One Hundred And Sixty )
109. Preparation of Competent Cells (Chemical or Electroporation Competency) | Fee: Rs 7,080.00 ( Rupees Seven Thousand Eighty )
110. Heat Shock or Electroporation of Cells with Ligation Mixture | Fee: Rs 7,080.00 ( Rupees Seven Thousand Eighty )
111. Recovery in SOC or LB Media | Fee: Rs 7,080.00 ( Rupees Seven Thousand Eighty )
112. Plating on Selective Media | Fee: Rs 3,540.00 ( Rupees Three Thousand Five Hundred And Forty )
113. Use of X-gal Plates to Distinguish Between Recombinant and Non-Recombinant Clones | Fee: Rs 7,080.00 ( Rupees Seven Thousand Eighty )
114. IPTG Induction of LacZ Marker | Fee: Rs 14,160.00 ( Rupees Fourteen Thousand One Hundred And Sixty )
115. Selection of White Colonies for Further Analysis | Fee: Rs 7,080.00 ( Rupees Seven Thousand Eighty )
116. Sterile Technique for Isolating Individual Colonies | Fee: Rs 2,360.00 ( Rupees Two Thousand Three Hundred And Sixty )
117. Inoculation in Liquid Culture for Amplification | Fee: Rs 2,360.00 ( Rupees Two Thousand Three Hundred And Sixty )
118. Storage of Clones in Glycerol Stocks at -80°C | Fee: Rs 4,720.00 ( Rupees Four Thousand Seven Hundred And Twenty )
119. Small-Scale (Miniprep) or Large-Scale (Maxiprep) Plasmid DNA Isolation | Fee: Rs 14,160.00 ( Rupees Fourteen Thousand One Hundred And Sixty )
120. Plasmid DNA Purification Using Spin Columns or Anion Exchange Chromatography | Fee: Rs 17,700.00 ( Rupees Seventeen Thousand Seven Hundred )
121. Determination of Plasmid Concentration and Purity | Fee: Rs 9,440.00 ( Rupees Nine Thousand Four Hundred And Forty )
122. Preparation of DNA for Sanger Sequencing or Next-Generation Sequencing | Fee: Rs 17,700.00 ( Rupees Seventeen Thousand Seven Hundred )
123. Primer Design for Specific Regions | Fee: Rs 7,080.00 ( Rupees Seven Thousand Eighty )
124. Submission of Samples to Sequencing Facility | Fee: Rs 4,720.00 ( Rupees Four Thousand Seven Hundred And Twenty )
125. Analysis of Sequencing Data for Verification of Cloning | Fee: Rs 17,700.00 ( Rupees Seventeen Thousand Seven Hundred )

Please contact on +91-8977624748 for more details