At NTHRYS Biotech Labs, we transform ideas into impactful CRISPR research experiences. Whether you're a budding undergraduate, an ambitious postgrad, a doctoral scholar, or a professional in the biotech or pharma sector, our CRISPR project offerings bridge academic exploration with real-world innovation. Dive into the dynamic field of genome editing and master advanced CRISPR techniques including CRISPR-Cas9, base editing, prime editing, and gene knockout workflows. Our programs offer a perfect blend of theoretical foundation, wet-lab experience, and in silico simulation, ensuring a 360-degree exposure to genetic engineering platforms. With expert mentoring, customizable modules, and real-time project tracking, you gain not just practical knowledge but also publication-ready data and industrial insights. Step into our labs located across Hyderabad, Bangalore, Chennai, and Trivandrum to explore plasmid design, target guide RNA construction, delivery systems (like electroporation or lipofection) , CRISPR efficiency analysis, and downstream validation methods like qPCR, Western blotting, or sequencing. Every participant receives certification, project documentation, and career mentoring. Our CRISPR projects are aligned with global trends in therapeutic gene correction, agri-genomics, antimicrobial resistance solutions, and synthetic biology. Join us to future-proof your career and become a part of the global gene-editing revolution. At NTHRYS, innovation meets mentorship—build your portfolio, publish your work, and grow with confidence in a world-class research environment.
1. CRISPR Orientation + Timeline of Genome Editing
(Dry Lab: Literature Survey, CRISPR Database Walkthrough)
5 Days
2. Target Gene Selection and Biological Relevance Framing
(Dry Lab: NCBI, Ensembl Search; Functional Relevance Matrix)
10 Days
3. gRNA Logic: Strand, Exon Selection, PAM Optimization
(Dry Lab: CHOPCHOP / CRISPRscan / Benchling Design)
15 Days
4. In Silico Off-Target Risk Management Plan
(Dry Lab: CRISPOR / Cas-OFFinder, Specificity Score Analysis)
20 Days
5. Project Proposal: Strategy Justification (Knockout/Knock-in/a/i)
(Dry Lab: Decision Matrix Design, Use-Case Simulation)
30 Days
6. Wet Lab Layout + CRISPR Protocol Integration Map
(Wet Lab: SOP Mapping, Reagent & Kit Selection)
45 Days
7. RNP/Plasmid Vector Selection + Delivery Modality Decision
(Dry Lab: Vector Map Design – SnapGene / Addgene Repositories)
2 Months
8. Execution of CRISPR Editing Round 1 (Initial Transfection)
(Wet Lab: gRNA + Cas9 RNP Assembly, Lipofection / Electroporation)
3 Months
9. Genomic DNA Isolation + Mutation Event Evaluation
(Wet Lab: Genomic DNA Extraction, PCR Amplification)
4 Months
10. Expression Analysis: Transcriptional Impact (qPCR / RT-PCR)
(Wet Lab: RNA Extraction, cDNA Synthesis, qPCR)
5 Months
11. Functional Analysis of Mutant vs Wild-Type
(Wet Lab: Western Blot, Cell Viability Assay / Fluorescence)
6 Months
12. Phenotype–Genotype Linking using Reporter Assays
(Wet Lab: GFP / Luciferase Reporter Imaging; Microscopy)
7 Months
13. Transcriptomic Shifts + Pathway Enrichment
(Dry Lab: RNA-Seq Data Analysis, DAVID, STRING, KEGG)
8 Months
14. Interactome + Pathway Rewiring Simulation
(Dry Lab: Cytoscape Visualization, Network Clustering)
9 Months
15. Integration of Case Studies + Real Disease Models
(Dry Lab: Comparative Study Models, Literature Integration)
10 Months
16. Risk + Regulatory Outlook + Bioethics in CRISPR
(Dry Lab: WHO & NIH Guidelines Review, Safety Framework Drafting)
11 Months
17. Drafting Patents, Publishing Data, Tech Transfer Planning
(Dry Lab: Scientific Writing, Reference Management, Patent Toolkits)
1 Year
Contact Us
