Please check Plant breeding genetics Internship details below.
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Traversing Diverse Plant breeding genetics Research Horizons: Specialized Research Methodologies and Varied Topics Unveiled
Research Methodologies focussed for Internship students under Plant breeding genetics:
Understanding and Improving Heat Stress Tolerance in Crops
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Heat stress is a significant challenge to agricultural productivity, impacting crop yield and quality. As global temperatures rise, developing strategies to enhance crop tolerance to heat stress is critical for ensuring food security. This research aims to dissect the genetic basis of heat tolerance in plants and leverage this knowledge to breed crops that can better withstand high temperature conditions.
Introduction
The increasing incidence of heat stress due to global warming poses a threat to food production worldwide. Understanding the physiological and molecular mechanisms of heat tolerance in crops is essential for breeding more resilient varieties.
Research Methodology
Phenotyping and Genotyping
- Conduct field trials and controlled environment studies to identify varieties with inherent heat tolerance.
- Use high-throughput phenotyping techniques to measure plant responses to heat stress, such as canopy temperature, leaf wilting, and photosynthetic efficiency.
- Apply genotyping tools to uncover genetic markers associated with heat tolerance traits.
Genetic and Genomic Analysis
- Employ genome-wide association studies (GWAS) and quantitative trait locus (QTL) mapping to identify genes linked to heat tolerance.
- Analyze transcriptomic, proteomic, and metabolomic data to understand the molecular responses of plants to heat stress.
- Utilize gene editing techniques, such as CRISPR/Cas9, to introduce or modify heat tolerance genes in target crops.
Breeding and Biotechnological Approaches
- Integrate traditional breeding methods with molecular breeding techniques to develop heat-tolerant crop varieties.
- Explore the use of transgenic approaches to introduce novel heat tolerance traits into crops.
- Develop and deploy diagnostic tools for the rapid selection of heat-tolerant genotypes in breeding programs.
Enhancing Drought Tolerance in Crops
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Drought stress significantly limits agricultural productivity and poses a growing threat to food security worldwide. Enhancing drought tolerance in crops involves understanding the complex physiological, biochemical, and genetic mechanisms that enable plants to withstand water scarcity. This objective seeks to identify key drought tolerance traits and genes, and use this knowledge to breed or engineer crops with improved resilience to drought conditions.
Introduction
With the increasing occurrence of drought conditions globally, there is an urgent need to develop crop varieties that can maintain yield and quality under water-limited environments. This research focuses on identifying and harnessing the genetic diversity and molecular mechanisms of drought tolerance.
Research Methodology
Identification of Drought Tolerance Traits
- Screen diverse plant species and varieties to identify those that exhibit superior performance under drought conditions.
- Analyze physiological traits such as root architecture, water use efficiency, and stomatal conductance that contribute to drought tolerance.
- Assess biochemical markers of drought stress response, including osmolyte concentration, antioxidant activity, and hormone levels.
Genetic and Genomic Analysis
- Utilize genome-wide association studies (GWAS) and quantitative trait loci (QTL) mapping to link drought tolerance traits to specific genetic markers.
- Conduct transcriptomic and proteomic analyses to identify genes and proteins that are differentially expressed under drought conditions.
- Employ gene editing and genetic engineering techniques, such as CRISPR/Cas9, to modify or insert genes associated with drought tolerance.
Breeding Strategies
- Integrate traditional breeding techniques with molecular markers to accelerate the development of drought-tolerant varieties.
- Implement marker-assisted selection (MAS) to efficiently select for desirable drought tolerance traits during breeding.
- Explore the potential of transgenic and gene editing approaches to introduce novel drought tolerance traits into target crop species.
Field Testing and Deployment
- Conduct field trials in multiple locations to evaluate the performance of developed varieties under real-world drought conditions.
- Analyze data on yield, quality, and resilience to identify the most promising drought-tolerant varieties for commercialization.
- Collaborate with local communities and agricultural stakeholders to deploy drought-tolerant crops and provide training on sustainable management practices.
Utilizing Evolutionary and Synteny Analysis for Effective Introgression Breeding Strategies in Rice
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Rice, as a staple food crop for more than half the world’s population, requires continuous improvement to meet increasing demand and overcome environmental challenges. This objective explores the use of evolutionary and synteny analysis to identify valuable genetic traits from wild relatives and efficiently introduce them into cultivated rice varieties through introgression breeding. This approach aims to enhance rice productivity, disease resistance, and environmental stress tolerance.
Introduction
The genetic diversity found in wild rice species represents a largely untapped resource for improving cultivated varieties. By understanding the evolutionary relationships and synteny (conserved gene order) among rice species, breeders can more effectively transfer beneficial traits to enhance crop resilience and yield.
Research Methodology
Evolutionary Analysis
- Collect and sequence genomes from a diverse range of wild and cultivated rice species.
- Analyze phylogenetic relationships to identify potential donors of beneficial traits.
- Study the evolutionary history of key traits to understand their development and distribution among species.
Synteny Analysis
- Identify conserved genomic regions between cultivated rice and its wild relatives.
- Map traits of interest to specific genomic regions with conserved synteny.
- Use comparative genomics to predict candidate genes responsible for beneficial traits.
Introgression Breeding
- Develop pre-breeding lines by crossing cultivated rice with wild relatives possessing desirable traits.
- Apply marker-assisted selection to identify progeny with successful trait introgression.
- Evaluate agronomic performance and trait stability of introgressed lines under various environmental conditions.
Deployment and Impact Assessment
- Conduct multi-location field trials to assess the performance of new rice varieties.
- Collaborate with farmers and agricultural extension services to promote the adoption of improved varieties.
- Monitor the impact of introgression breeding on rice productivity, disease resistance, and environmental stress tolerance in target regions.
Investigating the Genetic Basis of Salt Tolerance in Common Bean
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Salinity is a growing problem in agriculture, affecting soil health and reducing crop yield. The common bean, a major source of protein and nutrients for millions, is particularly sensitive to salt stress. This research aims to uncover the genetic factors that contribute to salt tolerance in the common bean, with the goal of breeding more resilient varieties that can thrive in saline conditions.
Introduction
Enhancing the salt tolerance of common bean is critical for sustaining its production in salt-affected areas. This project focuses on identifying salt-tolerant genes and alleles in common bean populations and elucidating the underlying molecular mechanisms of salt stress adaptation.
Research Methodology
Population Screening and Phenotypic Evaluation
- Screen diverse common bean populations under controlled salt stress conditions to identify salt-tolerant and salt-sensitive phenotypes.
- Measure key physiological traits associated with salt tolerance, such as ion accumulation, water retention, and growth metrics, under salt stress.
Genetic and Genomic Analysis
- Utilize genome-wide association studies (GWAS) to link phenotypic variation to specific genetic markers.
- Perform quantitative trait locus (QTL) mapping to identify genomic regions associated with salt tolerance traits.
- Analyze transcriptomic data to identify differentially expressed genes under salt stress conditions.
Molecular Characterization and Functional Validation
- Clone candidate genes identified through genetic and genomic analyses.
- Use gene editing technologies, such as CRISPR/Cas9, to validate the function of candidate genes in salt tolerance.
- Investigate the molecular pathways and networks involved in salt stress response and adaptation in common bean.
Breeding for Salt Tolerance
- Apply marker-assisted selection to incorporate salt tolerance traits into high-yielding common bean varieties.
- Evaluate the agronomic performance and stability of the newly developed salt-tolerant common bean lines under field conditions with varying salinity levels.
- Work with local farmers and agricultural stakeholders to deploy salt-tolerant common bean varieties in salt-affected regions, providing guidance on cultivation practices and management strategies.
Promoting the Use of Underutilized or Orphan Crops Through Integrative Genomics
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Underutilized or orphan crops hold great potential for contributing to food security, nutrition, and sustainable agriculture, especially in regions with challenging growing conditions. This objective seeks to leverage integrative genomics to unlock the genetic potential of these crops, enhancing their productivity, nutritional value, and resilience to environmental stresses.
Introduction
Despite their adaptability to local environments and nutritional benefits, many orphan crops lack the scientific attention given to major crops. By applying advanced genomics and breeding techniques, we can improve the agronomic traits of these crops and elevate their status in the global food market.
Research Methodology
Genomic Characterization
- Sequence the genomes of selected underutilized crops to establish a genetic baseline.
- Identify genetic diversity within and between populations to uncover traits that can be enhanced through breeding.
Trait Discovery and Gene Mapping
- Use phenotyping to identify desirable traits related to yield, nutrition, and stress tolerance.
- Employ genome-wide association studies (GWAS) and quantitative trait loci (QTL) mapping to associate these traits with specific genetic markers.
Gene Editing and Molecular Breeding
- Apply CRISPR/Cas9 and other gene-editing techniques to precisely improve traits of interest.
- Integrate traditional breeding with molecular tools to accelerate the improvement of underutilized crops.
Field Trials and Cultivar Development
- Conduct field trials to assess the performance of genetically improved lines under various environmental conditions.
- Select the most promising lines for further development and eventual release as new cultivars.
Outreach and Adoption
- Collaborate with local farmers and agricultural organizations to test and promote the adoption of improved varieties.
- Conduct workshops and training sessions to educate stakeholders about the benefits of cultivating and consuming underutilized crops.
Analyzing Intraspecies Variation in Repeat and Gene Content Within Crops Like Maize to Understand the Dynamics of Genetic Variation
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Maize, a crop with significant genetic diversity, provides an excellent model for studying intraspecies variation. This research aims to analyze the variation in repeat and gene content within maize to understand the dynamics of genetic diversity and its implications for crop improvement. Insights from this study could lead to the development of maize varieties with enhanced yield, nutritional value, and stress tolerance.
Introduction
Genetic variation within maize species is a valuable resource for breeding programs. This project focuses on understanding how differences in repeat and gene content among maize varieties influence their growth, development, and response to environmental stresses.
Research Methodology
Genomic Sequencing and Analysis
- Sequence the genomes of diverse maize varieties to catalog repeat elements and gene content.
- Analyze genomic data to identify patterns of genetic variation and their correlation with phenotypic traits.
Functional Genomics Studies
- Employ transcriptomics and proteomics to study the expression of genes within regions of high intraspecies variation.
- Investigate the functional implications of these variations on plant growth, development, and stress response.
Population Genetics and Evolutionary Analysis
- Analyze the genetic structure of maize populations to understand the evolutionary forces shaping intraspecies variation.
- Study the relationship between genetic diversity and adaptation to different environmental conditions.
Breeding and Genetic Improvement
- Use insights from genetic and functional analyses to identify target traits for improvement.
- Apply marker-assisted selection and genomic selection strategies to develop maize varieties with desired characteristics.
Applying Large-Scale Genomics to Improve Grain Yield, Stress Resilience, and Quality in Bread Wheat
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Bread wheat is a staple food crop with a complex genome that presents unique challenges and opportunities for genetic improvement. Utilizing large-scale genomics, this research aims to enhance wheat s grain yield, stress resilience, and grain quality, addressing global food security challenges and adapting to changing environmental conditions.
Introduction
As the demand for wheat grows alongside the global population, there is a pressing need to increase its productivity and resilience. Genomic approaches can unlock the potential for significant improvements by identifying key genetic determinants of desired traits.
Research Methodology
Genomic Sequencing and Characterization
- Sequence the genomes of a wide range of wheat varieties to capture genetic diversity.
- Analyze the genomic data to identify genetic markers linked to yield, quality, and stress tolerance traits.
Functional Genomics and Trait Mapping
- Conduct genome-wide association studies (GWAS) and quantitative trait loci (QTL) mapping to associate phenotypic traits with specific genomic regions.
- Use transcriptomics and proteomics to study gene expression and protein profiles related to key traits.
Gene Editing and Molecular Breeding
- Employ CRISPR/Cas9 and other gene editing technologies to introduce or modify traits in wheat.
- Integrate molecular breeding techniques with traditional breeding to expedite the development of superior wheat varieties.
Field Trials and Validation
- Evaluate the performance of genetically improved wheat lines under different environmental conditions through multi-location field trials.
- Analyze yield, quality, and resilience data to select the most promising varieties for commercial cultivation.
Leveraging Genebank Genomics to Bridge the Gap Between the Conservation of Crop Diversity and Plant Breeding
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Genebanks play a critical role in preserving agricultural biodiversity, housing vast collections of crop germplasm. By integrating genomics into genebank operations, this objective aims to enhance the utilization of stored genetic resources in plant breeding programs, facilitating the development of crops that are more productive, resilient, and adapted to diverse environmental conditions.
Introduction
The genetic diversity conserved in genebanks represents a valuable resource for addressing future agricultural challenges. However, the potential of these resources remains largely untapped. Genomic tools can unlock this potential, enabling targeted access to and use of genetic traits conserved in genebanks.
Research Methodology
Genomic Characterization of Genebank Collections
- Conduct high-throughput DNA sequencing to generate comprehensive genomic profiles of genebank accessions.
- Assess genetic diversity and identify unique alleles and traits present in the collections.
Data Integration and Access
- Develop databases and bioinformatics tools to manage and analyze genomic data from genebank collections.
- Implement user-friendly interfaces to facilitate access to genomic information by breeders and researchers.
Pre-breeding and Trait Discovery
- Use genomic information to identify candidate genes associated with desirable traits such as yield, nutrition, and stress tolerance.
- Collaborate with breeding programs to incorporate genebank-derived traits into modern cultivars through pre-breeding efforts.
Capacity Building and Collaboration
- Train genebank and breeding program staff in genomic techniques and data analysis to ensure effective use of genomic resources.
- Foster partnerships between genebanks, research institutions, and the agricultural industry to support the application of genebank genomics in plant breeding.
Other Objectives
- Enhanced Nutritional Quality: Improve the nutritional content of crops (e.g., vitamins, minerals, and proteins) to combat malnutrition.
- Climate Resilience: Develop crops that are more resilient to climate change effects, including increased temperatures, variable rainfall, and extreme weather conditions.
- Water Use Efficiency: Breed crops that require less water or can tolerate periods of water scarcity, essential for arid regions.
- Pest and Disease Resistance: Identify genetic markers for resistance against major pests and diseases to reduce reliance on chemical pesticides.
- Nitrogen Use Efficiency: Improve crops nitrogen uptake efficiency to reduce the need for synthetic fertilizers, minimizing environmental pollution.
- Carbon Sequestration: Develop crops with enhanced root systems capable of sequestering more carbon, contributing to climate change mitigation.
- Soil Health Improvement: Breed plants that contribute to soil health through enhanced root systems or nitrogen-fixation capabilities.
- Reduced Chemical Dependence: Create crops that require fewer chemical inputs (fertilizers, pesticides) for sustainable farming.
- Enhancing Photosynthesis Efficiency: Improve the photosynthesis process efficiency in crops to increase yield and reduce land use pressure.
- Post-Harvest Loss Reduction: Develop crops with longer shelf lives to reduce post-harvest losses and improve food security.
- Alternative Proteins Source: Enhance the yield and quality of legumes and other crops as sources of alternative proteins.
- Biodiversity Conservation: Utilize genetic diversity within crop wild relatives for breeding programs to enhance genetic pools and resilience.
- Biofortification: Increase the concentration of bioavailable nutrients in crops to address specific health issues (e.g., zinc, iron deficiency).
- Climate Adaptation Traits: Introduce traits for better adaptation to changing climates, such as early flowering or maturity to escape drought periods.
- Energy Crops Enhancement: Improve yield and quality of biomass for bioenergy crops, contributing to sustainable energy sources.
- Reducing Greenhouse Gas Emissions: Breed crops with reduced methane emissions or that require less tillage, contributing to lower agricultural GHG emissions.
- Herbicide Tolerance: Develop tolerance to herbicides in a way that minimizes environmental impact and resistance buildup.
- Efficient Phosphorus Use: Enhance phosphorus uptake and utilization to improve growth in phosphorus-poor soils.
- Salinity Tolerance: Introduce traits that allow crops to thrive in saline soils, expanding arable land.
- Enhanced Pollination Efficiency: Improve traits related to flower structure and timing to enhance natural pollination and yield.
- Sustainable Crop Rotations: Develop crops suited for sustainable rotations, improving soil health and pest control.
- Orphan Crops Development: Focus on underutilized crops with potential for improvement and significant local importance.
- Genome Editing for Precision Breeding: Utilize CRISPR and other genome editing technologies for precise trait improvement.
- Automated Phenotyping and Genotyping: Integrate advanced technologies for high-throughput plant phenotyping and genotyping to accelerate breeding.
- Blockchain for Genetic Resources: Implement blockchain technology for transparent and fair sharing of genetic resources and benefits.
- Increasing Seed Oil Content: Breeding oilseed crops with higher oil content for food and biofuel applications.
- Enhancement of Seed Storage Proteins: Improving the quality and quantity of storage proteins in seeds to enhance nutritional value.
- Developing Crops for Vertical Farming: Breeding crops specifically suited for vertical farming systems, optimizing space and resources in urban agriculture.
- Improvement of Root Architecture: Enhancing root depth and efficiency for better nutrient and water uptake, crucial for crop resilience.
- Heat Tolerance in Pollination Period: Developing crops that maintain pollination rates under high temperature conditions to ensure yield stability.
- <Synthetic Biology for Crop Improvement: Utilizing synthetic biology to engineer novel traits in crops for enhanced performance and resilience.
- Stress-Induced Metabolite Production: Breeding crops to produce beneficial metabolites in response to environmental stress, enhancing crop value and resilience.
- Improving Mechanical Harvestability: Breeding crops with traits that facilitate mechanical harvesting, reducing labor costs and improving efficiency.
- Enhancing Crop Aesthetics for Market Preferences: Developing crops with improved visual and taste qualities to meet market demands and increase consumer acceptance.
- Tailoring Crops for Non-Traditional Uses: Breeding crops for use in non-food products, such as bioplastics, pharmaceuticals, and cosmetics.
- Reducing Anti-Nutritional Factors: Breeding crops with lower levels of substances that interfere with the absorption of nutrients.
- Improving Forage Crops for Livestock: Enhancing the nutritional value and digestibility of forage crops to improve livestock health and productivity.
- Enhancing Ethanol Production Efficiency from Biomass: Breeding crops with optimized biomass composition for more efficient bioethanol production.
- Developing Climate-Smart Fruit and Vegetable Varieties: Creating varieties that maintain quality and yield under climate change scenarios.
- Breeding for Enhanced Micronutrient Mobilization to Edible Parts: Improving the mobilization of essential micronutrients from soil to the edible parts of crops.
- Optimizing Flowering Time for Extended Growing Seasons: Adjusting the flowering time of crops to extend the growing season and avoid climatic stress periods.
- Improving Crop Compatibility with Beneficial Microbes: Enhancing crop traits that promote symbiotic relationships with soil microbes for better growth and stress mitigation.
- Minimizing Crop Waste through Breeding: Developing crop varieties with traits that lead to minimal waste during processing and consumption.
- Developing Drought-Inducible Gene Expression Systems: Engineering crops with drought-inducible promoters for targeted gene expression under water scarcity.
- Precision Breeding for Allergen-Free Foods: Eliminating specific allergens from crops through targeted breeding to make foods safe for more consumers.
- Enhancing Secondary Metabolite Production for Natural Pesticides: Increasing the production of plant-derived compounds that can serve as natural pesticides.
- Breeding for Specific Cooking Qualities: Tailoring crops like rice and potatoes for specific texture and flavor profiles preferred in different cuisines.
- Enhancing Genetic Resistance to Crop Storage Pests: Developing crops with built-in resistance to pests that affect stored grains and seeds.
- Tailoring Plants for Regenerative Agriculture Practices: Breeding crops suited for regenerative agriculture, focusing on soil health, biodiversity, and ecosystem services.
- Developing Fast-Growing Tree Varieties for Reforestation and Bioenergy: Breeding faster-growing trees for sustainable timber, reforestation, and bioenergy purposes.
Fee Strctures for Plant Breeding Genetics Internship:
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Fee Reduction Chances:
- Group Reductions: Given for all durations for students who are joining in a group of 4 and above. There will be a considerable reduction given on total fee per head. Contact on below given number.
- Early Bird Reductions: Given for all students who are registering minimum three months before joining date. There will be 20% reduction given on total fee. Contact on below given number.
- MoU Reductions: Given for all students who are joining from institutions which has MoU with NTHRYS BIOTECH LABS will. There will be considerable reduction given on total fee. Contact on below given number.
- Toppers Reduction: Given for 3 months and above durations for all students who are top rankers in their institutions. Students can request fee reduction with the help of the head of institution (Principal, Not HOD) from the principals official email id. Contact on below given number.
- Economically Backward Class Reductions: Given for all durations to all students belonging to economically backward class can request for fee reduction. Contact on below given number.
Important Note: Candidates may apply only one type of reduction from the aforementioned list at any given time.
Contact via whatsapp on +91 - 9014935156 for reduction details.
Application Process
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- Our Academic Services department will confirm the application with in 10 mins to 1 hour.
Testimonials
VB. Bhavana View on Google
I have completed my 6 month dissertation in NTHRYS biotech labs. The lab is adequately equipped with wonderful, attentive and receptive staff. It is a boon to the students venturing into research as well as to students who would like to garner lab exposure. I had a pleasant experience at NTHRYS thanks to Balaji S. Rao Sir for his constant support, mettle and knowledge. I would also like to give special regards to Zarin Mam for teaching me the concepts of bioinformatics with great ease and for helping me in every step of the way. I extend my gratitude to Vijaya Mam, and Sindhu Mam for helping me carry out the project smoothly.
Durba C Bhattacharjee View on Google
I have just completed hands on lab trainings at NTHRYS in biotechnology which includes microbiology, molecular and immunology and had gained really very good experience and confidence having good infra structures with the guidance of Sandhya Maam and Balaji Sir.
Recommending to any fresher of biotechnology or microbiology field who wants to be expert before joining to
related industry.
Razia View on Google
Best place to aquire and practice knowledge.you can start from zero but at the end of the internship you can actually get a job that is the kind of experience you get here.The support and encouragement from the faculty side is just unexplainable because they make you feel like family and teach you every bit of the experiment.I strongly recommend NTHRYS Biotech lab to all the students who want to excel in their career.
Srilatha View on Google
Nice place for hands on training
Nandupandu View on Google
Very good place for students to learn all the techniques
Sadnaax View on Google
I apprenticed in molecular biology and animal tissue culture, helped me a lot for my job applications. Sandhya and Balaji sir were very supportive, very helpful and guided me through every step meticulously. Helped me learn from the basics and helped a lot practically. The environment of the lab is very hygienic and friendly. I had a very good experience learning the modules. Would recommend
Shivika Sharma View on Google
I did an internship in NTHRYS under Balaji sir and Sandhya maam. It was a magnificent experience. As I got hands-on experience on practicals and I was also provided with protocols and I learned new techniques too.This intership will help me forge ahead in life. The staff is very supportive and humble with everyone. Both sir and maam helped me with my each and every doubts without hesitation.
Digvijay Singh Guleria View on Google
I went for 2 months for different training programs at NTHRYS Biotech, had a fun learning experience. Everything was hands-on training and well organised protocols. Thank you Balaji sir and Sandhya mam for this life time experience.
Anushka Saxena View on Google
I’m a biotechnology student from Dy patil University mumbai and I recently completed my 6 months dissertation project at Nthrys Biotech Labs in Hyderabad. I had a great experience and I would highly recommend this lab to other students as well .
The first thing that I appreciated about Nthrys Biotech Labs was the friendly and supportive environment. Balaji sir and the staff Ragini and Sandhya ma’am were always willing to help me and they were always patient with my questions.
I also felt like I was part of a team and that I was making a real contribution to the companys research.
I learned a lot during my dissertation at Nthrys Biotech Labs not only academically but also personally . I had the opportunity to work on a variety of projects, which gave me a broad exposure to the field of biotechnology. I also learned a lot about the research process and how to conduct experiments.
In addition to the technical skills that I learned, I also developed my soft skills during my internship. I learned how to communicate effectively, how to work independently, and how to work as part of a team.
Overall, I had a great experience at Nthrys Biotech Labs and I would highly recommend this company to other students.
Once again I would like to render a big thank you to Balaji Sir and Vijayalakshmi ma’am for imbibing with all the knowledge along with helping me publish my research paper as well and its all because of them I scored unbelievably well in my final semester.
Nithin Pariki View on Google
Lab equipment and protocols are good, it gives good hands on experience for freshers.
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