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Plant Tissue Culture Training – 100 Categories & Crop-Specific Pipelines | NTHRYS

NTHRYS >> Services >> Academic Services >> Training Programs >> Plant Tissue Culture Training – 100 Categories & Crop-Specific Pipelines | NTHRYS

Plant Tissue Culture Training – From Lab Foundations to Crop-Specific Commercial Pipelines

This Plant Tissue Culture Training hub brings together 100 carefully structured categories covering lab setup, asepsis, media optimisation, core regeneration techniques, specialised systems and more than 60 crop-specific pipelines. Learners can explore end-to-end workflows for fruits, vegetables, ornamentals, medicinal plants, forestry and bioenergy crops, along with modules on hardening, nursery management, facility planning and commercial scale-up. Use the index below to jump into detailed category cards with modules, eligibility, delivery modes, enrollment policies and sector-wise applications.

Overview — PTC Lab Site Selection & Infrastructure Planning

This category helps you choose the right location and basic infrastructure blueprint for a plant tissue culture facility. You learn how to evaluate climate, utilities, logistics, contamination risks and regulatory constraints before committing to a site, and how to plan the footprint so it can expand from pilot to commercial scales. Emphasis is on practical checklists, risk assessment and integration of the PTC lab with nurseries and hardening units on the same campus.

Who can attend
  • Agri/biotech entrepreneurs planning new PTC facilities or expansions
  • Nursery owners integrating tissue culture into existing operations
  • Facility planners, architects & consultants working with PTC projects
  • Faculty/administrators setting up teaching or demonstration PTC labs
Learning Outcomes
  • Apply checklists for evaluating potential PTC sites (climate, access, utilities)
  • Identify zoning, regulatory and biosafety constraints before site finalisation
  • Assess risks related to power, logistics, flooding and contamination sources
  • Plan land/building footprint with clear expansion corridors and capacity targets
  • Document site visits and prepare a basic pre-commissioning site assessment report
Delivery Mode
  • Live online sessions on site selection logic, risk assessment and case studies
  • Optional on-site walk-throughs (Hyderabad region) for practical illustration
  • Template-based exercises for site comparison and scoring
  • Optional assignment: draft a site assessment note for a proposed PTC facility
Enrollment Policies
  • Seats confirmed after fee receipt & official confirmation email
  • One reschedule/deferral permitted within 90 days (subject to batch availability)
  • Certificate issued on completion of sessions and required assignment/quiz
  • Confidentiality of shared project/site details must be respected by all participants
Sectors — Where these modules are applied
  • Commercial PTC Labs & Nurseries: greenfield and expansion planning
  • Seed & Agri-Biotech Companies: in-house clean planting material units
  • Universities & Skill Centres: planning teaching, demo and incubation facilities
  • Agro-industrial Parks & Incubators: co-located PTC and nursery infrastructure design
Overview — Cleanroom Zoning & Functional Layout for PTC Labs

This category focuses on designing contamination-aware zones and functional layouts for plant tissue culture facilities. Trainees learn how to separate dirty, grey and clean areas, how to position media, inoculation and culture rooms for unidirectional flow, and how to integrate change rooms, gowning and support spaces. The emphasis is on practical, buildable layouts that reduce cross-traffic and support high-throughput, low-contamination operations.

Who can attend
  • PTC facility planners, architects and layout consultants
  • Entrepreneurs and nursery owners designing new PTC units
  • Lab managers upgrading from basic labs to cleaner, zoned facilities
  • Faculty/technical staff involved in planning teaching and demo PTC labs
Learning Outcomes
  • Define cleanroom classes and practical zoning schemes for PTC facilities
  • Design dirty, grey and clean corridors with unidirectional material and personnel flows
  • Lay out media, inoculation and culture rooms to minimise contamination risk
  • Position change rooms, gowning areas and airlocks for effective access control
  • Select appropriate finishes and surfaces for easy cleaning and long-term upkeep
Delivery Mode
  • Live online design sessions with sample PTC layout case studies
  • Optional on-site walkthrough of a functioning PTC facility (Hyderabad)
  • Sketch/CAD-based exercises to draft and critique functional layouts
  • Template pack: zoning diagrams, corridor flow maps and room adjacency charts
Enrollment Policies
  • Seats confirmed after fee receipt & official confirmation email
  • One reschedule/deferral allowed within 90 days (subject to batch availability)
  • Certificate issued on completion of sessions and layout-design exercise
  • Confidentiality of any shared floor plans or project details is mandatory
Sectors — Where these modules are applied
  • Commercial PTC Labs & Nurseries: greenfield and brownfield layout upgrades
  • Seed & Agri-Biotech Companies: zoned labs for breeder line and elite stock propagation
  • Universities & Training Centres: teaching/demonstration labs with clear zoning
  • Design & Consulting Firms: specialised cleanroom layout services for agri-biotech projects
Overview — Utilities Layout: Power, Water, HVAC & Air Handling

This category covers the backbone services that keep a plant tissue culture facility running reliably: electrical power and backup, process and potable water, RO/DI systems, HVAC and air handling units, pressure differentials and environmental monitoring. The focus is on how to size, place and connect utilities so that culture rooms see stable temperature, humidity, airflow and power — even in challenging field conditions — with clear SOPs for failures and cost-conscious operation.

Who can attend
  • PTC lab owners and project promoters planning new or upgraded utilities
  • Facility engineers, electricians & maintenance staff supporting PTC labs
  • Lab managers responsible for environment stability and uptime
  • Consultants and architects designing agri-biotech/TC facilities
Learning Outcomes
  • Estimate electrical loads for PTC labs and plan backup (DG/UPS/inverters)
  • Define water quality needs and select suitable RO/DI configurations
  • Understand HVAC & AHU concepts for cleanroom-grade culture rooms
  • Plan pressure differentials and airflow to support contamination control
  • Set up basic environmental monitoring and draft utility failure SOPs
Delivery Mode
  • Live online utility design & sizing sessions with worked examples
  • Optional on-site exposure to running PTC utilities (Hyderabad-based facility)
  • Spreadsheet exercises for load estimation, water consumption & energy costs
  • Template handouts: utility schematics, monitoring log sheets and failure checklists
Enrollment Policies
  • Seats confirmed after fee receipt & official confirmation email
  • One reschedule/deferral allowed within 90 days (subject to batch availability)
  • Certificate issued on completion of sessions and utility-planning exercise
  • Participants must respect confidentiality for any real facility data shared
Sectors — Where these modules are applied
  • Commercial PTC Labs & Nurseries: robust, low-downtime utility design
  • Seed & Agri-Biotech Companies: stable conditions for breeder/germplasm work
  • Universities & Skill Centres: reliable utilities for training and demo facilities
  • Agri-Industrial Parks & Incubators: shared utilities for multiple PTC tenants
Overview — Equipment Placement: Laminar Flow, Autoclave & Incubators

This category translates layout drawings into practical equipment positioning for plant tissue culture labs. Participants learn how to place laminar flow cabinets, autoclaves, incubators, culture racks and storage units so that workflows remain unidirectional, ergonomic and safe. Focus is on minimising contamination risk, avoiding heat and vibration conflicts, providing safe electrical access, and ensuring that operators can work efficiently in production-style PTC environments.

Who can attend
  • PTC lab managers and supervisors planning or revising equipment layouts
  • Entrepreneurs and nursery owners commissioning new PTC facilities
  • Facility engineers and safety officers supporting PTC operations
  • Faculty/technical staff responsible for student and trainee PTC labs
Learning Outcomes
  • Position laminar flow cabinets to support clean, ergonomic inoculation workflows
  • Locate autoclaves with safe access, drainage and load movement paths
  • Arrange incubators, culture racks and cold storage to avoid hotspots and vibration
  • Plan electrical points, panels and cut-offs with safety and maintenance in mind
  • Draft simple equipment layout maps and related SOPs for PTC facilities
Delivery Mode
  • Blended: online planning sessions + on-site equipment walk-throughs (Hyderabad)
  • Hands-on exercises: moving through mock layouts to test workflow and ergonomics
  • Layout critique sessions using sample PTC lab scenarios
  • Optional assignment: propose an equipment placement plan for a given floor plan
Enrollment Policies
  • Seats confirmed after fee receipt & official confirmation email
  • One reschedule/deferral allowed within 90 days (subject to batch availability)
  • Certificate issued on completion of sessions and layout-mapping exercise
  • All participants must follow lab safety and equipment-handling rules during visits
Sectors — Where these modules are applied
  • Commercial PTC Labs & Nurseries: production-line equipment placement
  • Seed & Agri-Biotech Companies: efficient layouts for breeder and elite material labs
  • Universities & Training Centres: safe, demonstrative setups for large student groups
  • Design & Consulting Firms: detailed equipment layouts as part of PTC project delivery
Overview — Media, Culture & Wash Room Layout Practicals

This category converts theory of media and glassware handling into real layouts. Trainees learn how to design media preparation and wash areas for quick turnaround, safe chemical storage, smooth drainage and minimal rework. Emphasis is on practical bench arrangements, segregation of clean/dirty activities and 5S-style organisation that supports high-throughput PTC pipelines.

Who can attend
  • PTC lab technicians and media room staff
  • Lab managers responsible for media and wash room operations
  • Entrepreneurs planning small-to-medium PTC units
  • Faculty setting up teaching labs with dedicated media/wash areas
Learning Outcomes
  • Plan media and wash room layouts that avoid cross-contamination
  • Design storage and benching that supports quick, error-free media prep
  • Implement safe drainage, effluent and spill management practices
  • Apply 5S/lean principles to keep media and wash areas organised
  • Prepare simple layout sketches and SOPs for routine operations
Delivery Mode
  • Blended: online layout planning + on-site media/wash room walkthroughs (Hyderabad)
  • Hands-on rearrangement exercises in existing rooms where possible
  • Template-based planning for storage, drainage and workflow zoning
  • Optional assignment: design a media/wash layout for a given case
Enrollment Policies
  • Seats confirmed post fee receipt & confirmation email
  • One reschedule/deferral within 90 days (subject to batch availability)
  • Certificate issued on completion of sessions and layout exercise
  • All participants must follow lab safety and chemical handling rules on-site
Sectors — Where these modules are applied
  • Commercial PTC Labs: media and wash rooms feeding high-volume culture pipelines
  • Seed & Agri-Biotech Units: in-house media prep for breeder and elite lines
  • Universities & Colleges: well-organised UG/PG teaching labs
  • Skill Centres: demonstration facilities for PTC process training
Overview — People & Material Movement Mapping in PTC Labs

This category teaches how people, media, explants and consumables should move through a PTC facility to keep contamination low and productivity high. Trainees learn to map flows from receiving to waste exit, identify cross-overs and dead zones, and redesign routes using simple diagramming tools. Emergency routes and visitor/audit paths are also covered.

Who can attend
  • PTC lab supervisors and production managers
  • Quality and EHS personnel responsible for facility risk assessments
  • Entrepreneurs optimising throughput in small/medium PTC labs
  • Students interested in industrial PTC facility management
Learning Outcomes
  • Map people, material and waste flows across PTC zones
  • Identify cross-traffic and contamination-prone choke points
  • Use simple spaghetti diagrams to redesign more efficient routes
  • Define visitor/audit routes that avoid disturbing production
  • Draft basic emergency evacuation and muster plans for PTC labs
Delivery Mode
  • Online sessions with flow-mapping case studies and templates
  • Optional on-site walk-throughs to draw real spaghetti diagrams
  • Group exercises to redesign flows for sample PTC layouts
  • Optional assignment: complete a movement map for your own/assigned lab
Enrollment Policies
  • Seat confirmation after fee receipt & institute email
  • One deferral permitted within 90 days (subject to slots)
  • Certification on completion of sessions and mapping exercise
  • Confidentiality for any real facility maps shared in class
Sectors — Where these modules are applied
  • Commercial PTC Labs: throughput and contamination control
  • Seed & Breeding Programs: controlled flows for elite material
  • Academic Labs: structured movement plans for large student batches
  • Incubators & Parks: multi-tenant PTC workflow planning
Overview — Facility SOPs: Entry, Gowning, Material Flow & Waste Exit

This category turns layout and flow diagrams into concrete, written SOPs governing how people, materials and waste move through a PTC facility. Participants learn to write clear, auditable procedures for entry, gowning, sterile transfers, waste handling and incident recording, along with systems for training and tracking staff competence.

Who can attend
  • PTC lab managers and QA/QC personnel
  • Entrepreneurs seeking audit-ready facility documentation
  • Faculty setting up structured PTC training labs
  • Senior technicians responsible for SOP implementation and training
Learning Outcomes
  • Draft entry, exit and access-control SOPs aligned with zoning
  • Define gowning/de-gowning procedures that support low contamination
  • Write SOPs for material receipt, quarantine, sterile transfer and waste exit
  • Set up basic deviation and incident documentation formats
  • Implement SOP training, competency checks and revision tracking
Delivery Mode
  • Online workshops on SOP structure, wording and version control
  • Sample SOP packs tailored to PTC facility operations
  • Role-play and checklist-based evaluation of SOP adherence
  • Optional assignment: draft or revise a set of facility SOPs for review
Enrollment Policies
  • Seats confirmed after fee receipt & confirmation email
  • One batch transfer allowed within 90 days (subject to schedules)
  • Certificate issued on completion of SOP drafting exercise
  • Confidentiality for any shared facility SOPs is mandatory
Sectors — Where these modules are applied
  • Commercial PTC Labs: client and certification-driven SOP frameworks
  • Seed & Breeding Units: controlled access to elite and breeder material
  • Academic Labs: standardised student access and safety procedures
  • Incubation Centres: common SOP templates for multiple resident startups
Overview — Preventive Maintenance & Calibration Plan for PTC Facilities

This category builds a disciplined maintenance and calibration backbone for PTC labs. It covers asset listing, preventive maintenance scheduling, routine calibration and qualification of key equipment, autoclave validation and the basics of logbooks, CMMS and vendor management so that culture rooms stay stable and failures are predictable, not random.

Who can attend
  • PTC lab managers and facility engineers
  • Maintenance technicians supporting PTC operations
  • QA/QC staff responsible for equipment qualification and records
  • Entrepreneurs who want reliable, low-downtime PTC facilities
Learning Outcomes
  • Create an asset register and identify critical PTC equipment
  • Design simple, practical preventive maintenance schedules
  • Implement routine calibration and basic IQ/OQ/PQ activities
  • Document autoclave validation using biological indicators
  • Use logbooks/CMMS and manage spares/vendors systematically
Delivery Mode
  • Live online sessions with PM and calibration planning exercises
  • Optional on-site exposure to maintenance & validation routines
  • Template set: PM schedules, calibration records, logbook formats
  • Optional assignment: build a PM/calibration plan for a sample lab
Enrollment Policies
  • Seats confirmed after fee receipt & official email
  • One reschedule/deferral allowed within 90 days (subject to availability)
  • Certificate issued on completion of PM/calibration planning task
  • Confidentiality for any facility/equipment data shared in exercises
Sectors — Where these modules are applied
  • Commercial PTC Labs: uptime-critical production facilities
  • Seed & Breeding Programs: long-term stability for elite material pipelines
  • Academic & Govt Labs: compliance-oriented maintenance and records
  • Agri-Biotech Parks: shared service models for multi-tenant PTC units


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