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NEKA-EH1971 Human CACYBP (Calcyclin Binding Protein) ELISA Kit

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₹ 37700^{Per Kit}

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Cat No: NEKA-EH1971

Standard Conc: 5000pg/mL

Sensitivity: 46.88 pg/mL

Assayrange: 78.13-5000pg/mL

Reactiontime: 3.5H

Volume: 96 wells

Detechtion Method: Sandwich

UniprotID: Q9HB71

Alternative Names: CACYBP, GIG5, S1A6BP, SIP, PNAS-17, calcyclin binding protein

Testing Principle:

Research Areas: Signal transduction;Metabolic pathway

Sample Type: serum, plasma, Tissue homogenate and Other biological samples

Product Status: Available

NEKA-EH1971 Human CACYBP (Calcyclin Binding Protein) ELISA Kit

Principle of Human CACYBP Calcyclin Binding Protein ELISA Kit Test

The Human CACYBP ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CACYBP in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CACYBP coated on a 96-well plate. Standards and samples are pipetted into the wells and CACYBP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CACYBP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CACYBP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Importance of the Test

CACYBP is known to play key roles in various physiological as well as pathological mechanisms in humans, including protein folding and degradation, neuronal signaling processes, and oncogenesis. Therefore, the accurate quantification of CACYBP levels can assist in the research and diagnosis of respective diseases or disorders associated with either overexpression or down-regulation of this protein.

List of Advantages

The kit has high sensitivity and excellent specificity for detection of human CACYBP.
No significant cross-reactivity or interference between human CACYBP and analogs was observed.
Convenient- The test can be completed in 3 hours.
High throughput- Up to 96 samples can be analyzed at a time.

Procedure to Perform the ELISA

Prepare all reagents, samples and standards;
Add 100μl standard or sample to each well. Incubate 2 hours at 37°C;
Remove the liquid of each well, don't wash;
Add 100μl Biotin-antibody to each well. Incubate 1 hour at 37°C;
Remove the liquid of each well, wash the microtiter plate;
Add 100μl HRP-avidin to each well. Incubate 1 hour at 37°C;
Remove the liquid of each well, wash the microtiter plate;
Add 90μl TMB Substrate to each well. Incubate 15-30 minutes at 37°C;
Add 50μl Stop Solution to each well. Check the color change and the change of color should be stopped;
Measure at 450nm immediately.

Results Analysis

Using a microplate reader, read the absorbance of each well at a wavelength of 450 nm. Firstly, calculate the average absorbance value for each set of duplicate standards and samples. Subtract the average zero standard absorbance from each. Plot a standard curve on log-log graph paper, with CACYBP concentration on the y-axis and absorbance on the x-axis. Draw the best-fit straight line through the standard points and it can be used to determine the concentration of samples.