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NEKA-EH5095 Human LFA-3/CD58 (Lymphocyte Function Associated Antigen 3) ELISA Kit

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₹ 37700^{Per Kit}

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Cat No: NEKA-EH5095

Standard Conc: 800pg/mL

Sensitivity: 7.5 pg/mL

Assayrange: 12.5-800pg/mL

Reactiontime: 3.5H

Volume: 96 wells

Detechtion Method: Sandwich

UniprotID: P19256

Alternative Names: Ag3, LFA-3, CD58

Testing Principle:

Research Areas: Enzyme & Kinase;

Sample Type: serum, plasma, Tissue homogenate and Other biological samples

Product Status: Available

NEKA-EH5095 Human LFA-3/CD58 (Lymphocyte Function Associated Antigen 3) ELISA Kit

Human LFA 3 CD58 Lymphocyte Function Associated Antigen 3 ELISA Kit Test Principle

In the Human LFA 3 CD58 Lymphocyte Function Associated Antigen 3 ELISA Kit Test, the LFA-3 antigen in samples binds to the biotin-coupled antibody specific for LFA-3, followed by incubation with streptavidin-horseradish peroxidase (HRP). This causes color change proportional to the amount of LFA-3 in the samples, providing a quantitative analysis.

Consumables in the ELISA Test

Microplate pre-coated with LFA 3/CD58 capture antibody
Biotinylated anti- LFA 3 /CD58 Detection antibody
Human LFA 3 /CD58 Standard
Streptavidin-HRP
Wash Buffer
Color Reagent A (H2O2)
Color Reagent B (TMB)
Stop Solution

Importance of this test

The test is crucial in detecting the presence and measuring the levels of LFA-3/CD58 in human biological samples. It aids in diagnosing diseases where LFA-3/CD58 expression is altered, such as autoimmune diseases and certain cancers.

List of Advantages

High specificity and sensitivity
Simple and convenient. Can be completed within a day
Quantitative results which can be easily analysed
A highly reproducible assay for accurate and consistent results

Detailed Procedure to perform this ELISA

Add samples and standards to the LFA-3/CD58 pre-coated microplate. Incubate for 2 hours at room temperature.
After washing, add the biotinylated anti- LFA 3 /CD58 detection antibody to the wells. Incubate for 1 hour.
Again wash the wells, followed by addition of HRP-conjugated streptavidin. Incubate for 30 minutes.
Add TMB substrate which turns blue in presence of HRP. Incubate for 15-30 minutes.
Add the Stop solution to stop the reaction. This will turn the blue color to yellow.
Measure the absorbance at 450 nm using a microplate reader. The intensity of yellow color is proportional to the concentration of LFA-3/CD58.

Results Analysis

Calculate the mean absorbance for each set of duplicate standards and samples. Subtract the average zero standard optical density. Plot a standard curve and derive the concentration of LFA-3/CD58 in the sample from this curve. If the levels of LFA-3/CD58 are higher or lower than normal, it may indicate a health issue.