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NEKA-EM5572 Mouse DNMT3L (DNA[cytosine-5]-methyltransferase 3-like) ELISA Kit

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₹ 37700^{Per Kit}

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Cat No: NEKA-EM5572

Standard Conc: 10ng/mL

Sensitivity: 0.1 ng/mL

Assayrange: 0.16-10ng/mL

Reactiontime: 3.5H

Volume: 96 wells

Detechtion Method: Sandwich

UniprotID: Q9CWR8

Alternative Names: DNMT3L, DNA methyltransferase 3 like

Testing Principle:

Research Areas: Infection immunity;Hormone metabolism;

Sample Type: serum, plasma, Tissue homogenate and Other biological samples

Product Status: Available

NEKA-EM5572 Mouse DNMT3L (DNA[cytosine-5]-methyltransferase 3-like) ELISA Kit

Principle of Mouse DNMT3L DNA Cytosine 5 Methyltransferase 3 Like ELISA Kit Test

This ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Mouse DNMT3L (DNA Cytosine-5 Methyltransferase 3-like) protein in cell culture supernatant, serum, plasma and other biological fluids.

Consumables in the ELISA Test

Micro ELISA Plate
DNMT3L Standard
Standard Diluent
DNMT3L Detection Reagent
Assay Diluent A
Assay Diluent B
Stop Solution
Positive and negative controls
Wash Buffer

Importance of this Test

DNMT3L is involved in de novo methylation of DNA, chromatin condensation and embryonic development. Any alterations in the expression and function of DNMT3L are associated with various diseases such as cancer. Its measurement can help in understanding disease pathogenesis and conducting research in epigenetic regulation.

List of Advantages

The ELISA kit has high sensitivity and excellent specificity for detection of DNMT3L, without cross-reactivity with other analogues.
Tests are quick, affordable and can analyze a large number of samples at once.
No significant homology with other cytosine methyltransferases.
Data gathered from these tests can contribute greatly to scientific research.

Detailed Procedure to Perform this ELISA

Add standards and test samples to the wells of the micro ELISA Plate.
Add the prepared Detection Reagent A working solution.
Shake and incubate at 37°C for 1 hour.
Wash the micro ELISA plate and add the prepared Detection Reagent B working solution.
Incubate at 37°C for 30 minutes.
Wash the micro ELISA plate again and add the substrate solution.
Incubate at 37°C for 15 minutes.
Add the stop solution.
Read the OD value at 450 nm.

Results Analysis

The standard curve is plotted with DNMT3L concentration on the x-axis and the OD value on the y-axis. The DNMT3L concentrations in the samples are then determined by comparing the OD of the samples to the standard curve.