Principle of Mouse HDAC1 Histone Deacetylase 1 ELISA Kit Test
The Mouse HDAC1 Histone Deacetylase 1 ELISA Kit is based on the principle of Enzyme Linked Immunosorbent Assay (ELISA) which involves antigen-antibody interactions. This test uses pre-coated wells with the HDAC1 antigen and antibodies conjugated with an enzyme. When the sample is added into the well, any HDAC1 protein present will undergo a sandwich ELISA, binding with the pre-coated antigen and the enzyme-linked antibody. After a series of washings, a substrate is added, which react with the enzyme to produce a color visible to the naked eye. The intensity of this color is proportional to the concentration of the HDAC1 protein in the sample.
Importance of the Mouse HDAC1 Histone Deacetylase 1 ELISA Kit Test
HDAC1 plays a pivotal role in the control of gene expression. Its irregular activities are connected with several diseases including cancer, thereby making it an important biological marker. This ELISA kit provides a quantitative and systematic way to study HDAC1 function, understand its role in various diseases, and identify potential HDAC1-targeting therapeutic agents.
Advantages of Mouse HDAC1 Histone Deacetylase 1 ELISA Kit Test
Easy to use: no specialized knowledge or equipment required
Avoids complicated purification steps
High sensitivity and specificity
Replicable and reliable results
Procedure to Perform the ELISA Test
Preparation: Keep all reagents at room temperature for 30 minutes before use. Prepare the standard by diluting the lyophilized standard with the sample dilution buffer.
Adding samples: Apply standard, control or sample to each well, then add the Biotin conjugated Antibody. Cover with an adhesive strip and incubate for 2 hours at room temperature.
Washing: Remove the liquid of each well, add 400 µL of Wash Buffer, then remove the Wash Buffer. Repeat the washing process twice more.
Enzyme Reaction: Add 100 µL of HRP-conjugate to each well. Cover with an adhesive strip and incubate for 30 minutes at room temperature.
Substrate Reaction: Add 90 µL of Substrate Solution to each well. Cover with a new adhesive strip and incubate for 15 minutes at room temperature.
Stop Reaction: Add 50 µL of Stop Solution to each well. Read immediately at 450 nm.
Result Analysis
The optical density of the samples is read using a microplate reader at 450 nm. The standard curve is drawn by plotting the optical density against the corresponding concentrations of the standards and the HDAC1 concentrations in the samples are determined from the standard curve. Higher levels of HDAC1 in the sample are indicated by a higher optical density.
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