Alternative Names: EMILIN1, EMI, EMILIN, gp115, elastin microfibril interfacer 1
Testing Principle:
Research Areas: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;
Sample Type: serum, plasma, Tissue homogenate and Other biological samples
Product Status: Available
NEKA-ER4992 Rat EMILIN1 (Elastin Microfibril Interface Located Protein 1) ELISA Kit
Principle of the Test
The Rat EMILIN1 Elastin Microfibril Interface Located Protein 1 ELISA Kit is a solid phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The samples and standards are pipetted into wells with an immobilized polyclonal antibody specific for rat EMILIN1. The captured EMILIN1 from the samples reacts with a biotinylated detection polyclonal antibody, which is recognized by a streptavidin-peroxidase complex. A substrate solution is then added to result in color change that varies in intensity proportionally to the amount of EMILIN1 present in the sample. The color development is stopped and the intensity of the color is measured.
Importance of the Test
The accurate quantitative measurement of rat EMILIN1 levels in biological samples is crucial for monitoring disease management. EMILIN1 is known to play critical roles in cell adhesion, tissue architecture, and blood vessel formation. A deficiency is linked to disorders such as heart diseases and EDS syndrome, therefore, an ELISA test to detect its levels gives researchers valuable insights into various human health conditions.
Advantages of the Test
Highly sensitive and specific measurement of Rat EMILIN1.
Can be used with serum, plasma, and cell culture supernatants.
Assay time is less than 4 hours and can be performed in a 96-well format, allowing for high-throughput sampling.
ELISA kit includes all needed components.
Procedure to Perform the Test
Add the samples and standards to the wells.
Incubate at room temperature for 2.5 hours.
Remove the liquid from all wells.
Add biotinylated detection antibody to all wells - this binds to the EMILIN1.
Incubate at room temperature for 1 hour.
Remove the liquid from all wells and add streptavidin-horseradish peroxidase to all wells.
Incubate at room temperature for 45 minutes.
Remove the liquid from all wells and wash the plate 3-4 times with wash buffer.
Add TMB substrate solution to all wells and incubate for 30 minutes in dark conditions.
Add Stop Solution to each well to stop the reaction.
Read the optical density (OD) of each well at 450 nm using an ELISA reader within 10 minutes of adding Stop Solution.
Result Analysis
Calculate the mean absorbance (OD) value for each set of duplicate standards and samples. Subtract the mean OD of the blank well from all readings. Construct a standard curve by plotting the mean OD for each standard and draw a best fit curve through these points. Use this curve to determine the concentration of EMILIN1 in each of your samples.
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