Click
Drag
NTHRYS WHATSAPP
NTHRYS LOGO Welcome Interface Environment Research Products Academics About Contact
NTHRYS LOGO

Stem Cell Transformations Job Oriented Training

Info
Testimonials
FAQs
Placements
Updates

NTHRYS provides Stem Cell Transformations Job Oriented Training for interested candidates at its Hyderabad facility, Telangana. Please refer below for more details including Fee strctures, Eligibility, Protocols and Modules etc.,. Please do call / message / whatsapp for more details on 9014935156 [India - +91]

Eligibility: BSc / BTech / MSc / MTech / MPhil / PhD in any Life Sciences studying or completed students

Protocols / Techniques Covered

    Molecular Biology & rDNA Technology

  1. DNA Extraction from Human Blood
  2. DNA Extraction from Bacteria
  3. DNA Extraction from Plant Leaf
  4. DNA Extraction from Chicken Liver
  5. Primer designing using Bioinformatics Tools
  6. Optimization of PCR parameters
  7. PCR
  8. Agarose Electrophoresis using 1 - 10 Kbp ladder
  9. Extraction & purification of amplified DNA from Agarose gels using spin columns
  10. Cultivation of pUC 18 vector bearing bacterial strain
  11. Plasmid [pUC 18] isolation
  12. Restriction digestion digestion of pUC18 vector using EcoRI
  13. 5` End DNA modification of restriction digested plasmid sample [Addition of Poly Ts]
  14. TA Cloning [PCR Product and sample obtained above]
  15. DNA ligation
  16. Cultivation of DH5 alpha cells and Competent cell preparation using cultivated DH5 alpha cells
  17. Bacterial Transformation [using competent cells and cloned vector obtained above]
  18. Blue white screening [checking for the transformed colonies]
  19. 5 Days Duration - [Protocols 1, 5, 6, 7 & 8 are covered]

    10 Days Duration - [Protocols 1, 2, 3, 5, 6, 7 & 8 are covered]

    20 Days Duration - [Protocols 1, 2, 3, 4, 5, 6, 7 & 8 are covered]

    1 Month Duration - [Protocols 1 to 13 are covered]

    45 Days Duration - [All the above mentioned protocols are covered]

    Animal Tissue Culturing (Stem Cells Cultivation)

    Animal cells are more difficult to culture than microorganisms because they require many more nutrients and typically grow only when attached to specially coated surfaces. Despite these difficulties, various types of animal cells, including both undifferentiated and differentiated ones, can be cultured successfully.

    NTHRYS Biotech Labs has introduced Animal Tissue Culture Training in the year 2009 to interested students / scholars. Please refer below for complete details:

    Animal Tissue Culture Training Module

     

    Module

    Protocols List

    Module - I

    Protocols covered under this Module - I:

    1. Preparation of Animal Tissue Culture Media
      1. Stock Solutions
      2. Eagle's Base (10X)
      3. Amino Acid Mixture (100X)
      4. Vitamin Mixture (100X)
      5. Ferric Nitrate (1000X)
      6. DMEM (1X) - (Working Medium)
      7. PBS (Phosphate Buffered Saline)
    2. Cultivation of Human Bone Marrow Stem Cells
    3. Cultivation of Human Umbilical Cord Blood Stem Cells
    4. Cultivation of Human Cardiomyocytes
    5. Cultivation of HeLa Cells
    6. Passaging
    7. Preparation and Use of Conditional Media [ Using Human Cardiomyocytes ]
    8. Staining
      1. Acid Phosphatase Staining
      2. Staining using Periodic Schiff's Reagent

    Module - II

    Protocols covered under this Module - II:

    1. Separation of Fetal Human Serum [FHS] from Cord Blood.
    2. Collection of Cardiomyocytes source and isolation of Cardiomyocytes.
    3. Preparation of conditional media from cardiomyocytes cultivation.
    4. Collection & Cultivation of Human Bone Marrow stem cells.
    5. Utilization of Cardiomyocyte Conditional media to transform Bone Marrow stem cells to cardiomyocytes.
    6. Utilization of Cardiomyocyte conditional media to transform Cord blood stem cells to cardiomyocytes
    7. Qualitative analysis for confirmation of Cord Blood Stem cells
    8. Qualitative analysis for confirmation of Human Bone Marrow stem cells
    9. Qualitative analysis for confirmation of Cardiomyocytes
    10. Qualitative analysis for confirmation of Cardiomyotes transformed from Cord blood stem cells
    11. Qualitative analysis for confirmation of Cardiomyocytes transformed from Human Bone Marrow stem cells

    Module - III

    Protocols covered under this Module - III:

    1. Collection, Cultivation & preservation of Cord blood stem cells.
    2. Collection & Preservation of Human Cord Blood.
    3. Isolation, Cultivation & Confirmation of Human Liver Cell Lines
    4. Isolation, Cultivation & confirmation of Human Pancreatic Cell Lines
    5. Isolation, Cultivation & confirmation of Human Alveolar Cell Lines
    6. Isolation, Cultivation & Confirmation of Green monkey kidney cell lines
    7. Isolation, Cultivation & Confirmation of Human Neural Cells
    8. Isolation, Cultivation & Confirmation of Organ specific stem cells
      1. Neural Stem Cells
      2. Bone Marrow Stem Cells

    Durations & Fee Structures

    5 Days Duration - Module - 1 [Protocols 1, 2, 6 & 8]

    10 Days Duration - Module - 1 [Protocols 1, 2,3,4, 6 & 8]

    20 Days Duration - Module - 1 [All Protocols] & Module - 2 [Protocols 1,2,3 & 4]

    1 Month Duration - Module - 1 [All Protocols] Module - 2 [All Protocols]

    45 Days Duration - Module - 1 [All Protocols] Module - 2 [All Protocols], Module - 3 [All Protocols]

    Immunotechnology or Immunology

  20. Extraction of IgG [Immunoglobulin G] from plasma / serum
  21. Purification of extracted Immunoglobulins [Using Dialysis process]
  22. Pepsin digestion and purification of digested IgG
  23. Preparation of Antigens for Immunizations [including Adjuvant selection strategies]
  24. SDS PAGE
  25. ELISA [Sandwich ELISA - Qualitative]
  26. RID
  27. DID [Ouchterlony]
  28. Immunization of Mice or Rabbit
  29. Plant Tissue Culturing

  30. Preparation of media and stock solution
  31. Preparation of Explants
  32. Callus initiation and Maintenance [In Potato]
  33. Shoot & Root Induction in potato
  34. Microbiology

  35. Isolation & Screening of soil microorganisms
  36. Microbial stainings [Normal & Gram Staining]
  37. Bacterial Motility test
  38. Catalase Test
  39. Mannitol Salt Agar Test
  40. Blood Agar plates assay
  41. Optochin sensistivity test
  42. Bacitracin sensistivity test
  43. CAMP Test
  44. Bile esculin agar test
  45. Nitrate broth test
  46. Spirit blue agar test
  47. Starch hydrolysis test
  48. Coagulase test
  49. Oxidase test
  50. Sugar broth with durham's tubes test [Carbohydrate metabolism test]
    Glucose Test
    Sucrose Test
    Mannose Test
  51. Methyl Red Voges Proskauer Test [MRVP Test]
  52. Kliger's Iron Test
  53. MacConkey Agar Test
  54. Simmon's Citrate Test
  55. Ureas Test
  56. Sulfur Indole motility media test
  57. Indole Test
  58. Kliger's Iron Test
  59. Bioinformatics

    Basic Bioinforrmatics Training Module

    Module I

    Theory

    Practical

    Tools

    History

    Biological Databases

    NCBI,MMDB,EMBL,DDBJ,SwissProt

    Origin

    Structure DB

    PDB,CATH,SCOP,InterproScan,Signal Scan

    Scope of Bioinformatics

    Importance of Tools

    N/A

    Origin of Tools

    Sequence DB’s

    Scan,Prosite,Prodom, MotifScan,PFam

    Sequence File Formats

    Types

    Genebank file format,FASTA format,EMBL format,UniprotKB/Swiss-Prot format, PIR/NBRF format

    Module II

    Application of Bioinformatics

    Gene Prediction & Functional Analysis

    ORF finder, GeneScan,GeneMark,Webgene

    Sequence Comparison

    EXPASy, EMBOSS

    BLAST,Clustalw,DIALIGN

    Structure File Formats

    Repeat detection

    Repeat Masker, dnadot

    General Introduction to Molecular Biology

    Hydrophobicity

    Protparam

    Restriction Site Mapping

    Restriction site Detection

    Webcutter,NEBCutter

    Visualiztion  Software

    System Biology Vs /w

    RasMol,SPDBV,JMol,Cn3D

    Phylogenetic Analysis

    Evolutionary Relationship

    Phylogeny,HHperd, Biology workbench

    Bioinformatics Dogma

    Thermodynamics

    ProTherm

    Minor Project Concerning the concepts learnt

    SAS

    Below module is also provided Online. [ SAS Online Training ]

    BASE SAS

  60. Navigation of SAS Software Tool
  61. STEPS For Writing the SAS Program
  62. SORT PROCEDURE
  63. PRINT PROCEDURE
  64. SUBSETTING data(OBS) by using WHERE Condition with various OPERATORS
  65. Controlling which OBSERVATIONS to be read from INPUT to OUTPUT dset
  66. Controlling which VARIABLES to be read from INPUT to OUTPUT dset
  67. Creating Multiple Output Datasets from One Single INput Dataset
  68. FORMATS
  69. RETAIN STATEMENT
  70. SUM ASSIGNMENT STATEMENT
  71. BY GROUP PROCESSING
  72.  

    Reading Data from Raw Data Files and Creating SAS Dataset

  73. DATALINES STATEMENT
  74. LIST INPUT STYLE
  75. Reading Multiple Observations Per Record DOUBLE TRAILING @@ on INPUT statement
  76. When Missing Values at the end of the record
  77. Reading Multiple Records Per Observation
  78. COLON MODIFIER
  79. SINGLE TRAILING (@) ON INPUT STATEMENT
  80. COLUMN INPUT STYLE / FIXED WIDTH INPUT STYLE
  81. FUNCTIONS

  82. CHARACTER FUNCTIONS
  83. COMPRESS Function
  84. CONCATENATE FUNCTION / CONCATENATE OPERATOR
  85. CONCATENATE FUNCTION
  86. SUBSTRING Function
  87. FIND Function
  88. INDEX Function
  89. SCAN Function
  90. TRANWRD Function
  91. NUMERIC FUNCTIONS 
  92. MATHEMATICAL FUNCTIONS
  93. DATE TIME FUNCTIONS
  94. Data Type Conversion Functions
  95. Report Procedure / Summary Procedures

  96. Frequency Procedure
  97. MEANS PROCEDURE
  98. Dealing with MISSING values in PROC MEANS
  99. PROC SUMMARY
  100. PROC UNIVARIATE
  101. Tabulate Procedure
  102. REPORT PROCEDURE
  103. Creating a Detailed Report by enhancing output results
  104. Creating a SUMMARIZED Report by using GROUP USAGE
  105. DISPLAY USAGE
  106. Creating new computed variables from existing variables using COMPUTE BLOCK
  107. ACROSS USAGE
  108. REPORT BREAKS
  109. Combining Sas Datasets

  110. Concatenation
  111. Merging of datasets (horizontally)
  112. ONE to ONE READING and ONE to ONE MERGING
  113. MATCH MERGING
  114. UPDATING & MODIFYING
  115. MISSINGCHECK / NOMISSINGCHECK Options
  116. Additional Transformations

  117. Contents Procedure
  118. Selecting the VARIABLE RANGE LIST using
  119. Proc Sort with NODUPKEY and NODUPRECS
  120.       4.   TRANSPOSE

  121.  ARRAYS
  122.  Do While / Do Until
  123. Append Procedure
  124.  DEBUG in SAS
  125.  ADVANCED SAS

       SAS SQL

  126. Enhancing the output results using PROC SQL
  127. GROUP BY Statement
  128. SUBQUERIES
  129. DISTINCT Keyword
  130. VALIDATE Keyword
  131. Joins
  132. SET OPERATORS
  133. DELETING ROWS
  134. MACROS

  135. Macro Introduction
  136. Macro Variable Storage
  137. Macro Options
  138. Macro Parameters
  139. Additional Macro Programs
  140. Creating MacVars using Proc Sql
  141. Creating a Macro Variable During Data Step Execution
  142. Symget Function to Search for Macro Variable Value
  143. Auto Call Macros
  144. Macro Debugging System Options
  145. Storing Stored Compiled Macr Program to a Permanent Library

Fee Structures for Stem Cell Transformations Job Oriented Training

Fee details in Rs per student
Fee 3 Months 4 Months 5 Months 6 Months
Individual 151600 298400 417500 494300
Group 2 - 4 144100 201000 392800 508300
Group 5 - 7 142600 198900 388700 503000
Group 8 - 10 141100 196800 384600 497600


Note: NTHRYS currently operates through three registered entities: NTHRYS BIOTECH LABS (NBL), NTHRYS OPC PVT LTD (NOPC), and NTHRYS Project Greenshield (NPGS).

NTHRYS CONSORTIUM

NBL NOPC NPGS
Updates
Industrial

Basic

Academic

Terms | Refund and Cancellations | Privacy Policy | Shipping Policy | Retruns Policy
Website Designed, Programmed and Maintained
by
Balaji S Rao


Web

Chat
Contact

Us
Download

PDF
Placements